Hybrid inflorescences derived from gamma-fusion of Arabidopsis thaliana with Bupleurum scorzonerifolium

文献类型： 外文期刊

第一作者： Xia, Guangmin

作者： Xia, Guangmin;Peng, Zhenying

作者机构：

关键词： Arabidopsis thaliana (L.) Heynh; Bupleurum scorzonerifolium Willd; Gamma ray (gamma-ray) irradiation; Asymmetric somatic hybridization; Hybrid inflorescence and flower

期刊名称：PROTOPLASMA （ 影响因子：3.356； 五年影响因子：3.298 ）

ISSN： 0033-183X

年卷期： 2012 年 249 卷 1 期

页码：

收录情况： SCI

摘要： In our early experiments, a variety of Bupleurum scorzonerifolium-like somatic hybrid plants were obtained from protoplast fusion between Arabidopsis thaliana and UV-treated/untreated B. scorzonerifolium. To compare the effects of UV and gamma-ray irradiation on the B. scorzonerifolium partner and obtain Arabidopsis-like hybrids, we designed a novel combination of somatic hybridization between A. thaliana and B. scorzonerifolium. Before protoplast isolation and fusion, the suspension cells of B. scorzonerifolium were irradiated by gamma ray (Co-60, 50 Gy with 1.3 Gy min(-1)). Both parental protoplasts lost regeneration capacity, but over 100 somatic hybrids restored the capacity and developed to Arabidopsis-like inflorescences and flowers with some characteristics of B. scorzonerifolium. Some hybrid flowers showed yellow sepal, petal, or carpel, whose color was similar to the petal of B. scorzonerifolium; the others had silique of Arabidopsis with angularity of B. scorzonerifolium, and their parts possessed five stamens, the same as B. scorzonerifolium. Cytological analysis showed that three hybrids had Arabidopsis-like karyotypes. Random Amplified Polymorphic DNA (RAPD) and Simple Sequence Repeats (SSR) profiles revealed that both parental fragments were amplified from these hybrids. These results indicated chromatin introgression from B. scorzonerifolium to A. thaliana, which may be related to the complementation of hybrid inflorescence and flower generation.

分类号： Q24

  • 相关文献
作者其他论文 更多>>

微信小程序