A simple and sensitive competitive bio-barcode immunoassay for triazophos based on multi-modified gold nanoparticles and fluorescent signal amplification

文献类型： 外文期刊

第一作者： Zhang, Chan

作者： Zhang, Chan;Jiang, Zejun;Jin, Maojun;Chen, Ge;Cao, Xiaolin;Cui, Xueyan;Zhang, Yudan;Li, Ruixing;Wang, Jing;Zhang, Chan;Jiang, Zejun;Jin, Maojun;Chen, Ge;Cao, Xiaolin;Cui, Xueyan;Zhang, Yudan;Li, Ruixing;Wang, Jing;Du, Pengfei;Du, Pengfei;Du, Pengfei;Abd El-Aty, A. M.;Abd El-Aty, A. M.

作者机构：

关键词： Immunoassay; Fluorescence; Bio-barcode; Gold nanoparticles; Triazophos

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.558； 五年影响因子：6.228 ）

ISSN： 0003-2670

年卷期： 2018 年 999 卷

页码：

收录情况： SCI

摘要： A simple and highly sensitive immunoassay based on a competitive binding and bio-barcode amplification was designed for detection of small molecules, triazophos. The gold nanoparticles (AuNPs) were modified with monoclonal antibodies and 6-carboxyfiuorescein labeled single-stranded thiol-oligonucleotides (6-FAM-SH-ssDNAs); the fluorescence of 6-FAM was quenched by AuNPs. Ovalbumin-linked haptens were coated on the bottom of microplate to compete with the triazophos in the sample for binding to the antibodies on the AuNP probes. The fluorescence intensity was inversely proportional to analyte concentration. Parameters of AuNP probes preparation and immune reaction were optimized. At the optimal conditions, the salting process was shortened to 1 h and 166 +/- 9 ssDNAs were loaded onto a single AuNP. The competitive fluorescence bio-barcode immunoassay was performed on water, rice, cucumber, cabbage and apple samples. The linear range of the method was 0.01-20 mu g L-1 and the limit of detection (LOD) was 6 ng L-1. The recovery and relative standard deviations (RSDs) ranged from 85.0 to 110.3% and 9.4-17.4%, respectively. Good correlations were obtained between the results of the developed method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). In conclusion, it is suggested that the competitive fluorescent bio-barcode immunoassay had the potential to be used as a sensitive method for detection of a variety of small molecules in various samples. (C) 2017 Elsevier B.V. All rights reserved.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序