An electrochemical biosensor for sensitive detection of live Salmonella in food via MXene amplified methylene blue signals and electrostatic immobilization of bacteriophages

文献类型： 外文期刊

第一作者： Deng, Tingliu

作者： Deng, Tingliu;Zeng, Qin;Deng, Chunyan;Wu, Wuming;Zhou, Jingjing;Zhou, Jingjing;Wang, Heye;Wang, Heye

作者机构：

关键词： Phages; Oriented immobilization; MXene; Nanostructured electrodes; Modified glassy carbon electrode; Differential pulse voltammetry; Salmonella

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.3； 五年影响因子：5.0 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 9 期

页码：

收录情况： SCI

摘要： A novel bacteriophage-targeted electrochemical biosensor designed for accurate and quantitative detection of live Salmonella in food samples is presented. The biosensor is simply constructed by electrostatic immobilizing bacteriophages on MXene-nanostructured electrodes. MXene, renowned for its high surface area, biocompatibility, and conductivity, serves as an ideal platform for bacteriophage immobilization. This allows for a high-density immobilization of bacteriophage particles, achieving approximately 71 pcs mu m(-2). Remarkably, the bacteriophages immobilized MXene nanostructured electrodes still maintain their viability and functionality, ensuring their effectiveness in pathogen detection. Therefore, the proposed biosensor exhibited enhanced sensitivity with a low limit of detection (LOD) of 5 CFU mL(-1). Notably, the biosensor shows excellent specificity in the presence of other bacteria that commonly contaminate food and can distinguish live Salmonella from a mixed population. Furthermore, it is applicable in detecting live Salmonella in food samples, which highlights its potential in food safety monitoring. This biosensor offers simplicity, convenience, and suitability for resource-limited environments, making it a promising tool for on-site monitoring of foodborne pathogenic bacteria.

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