Conservative gene recognition-promoted electrochemical strategy for sensitive detection of Ralstonia Solanacearum phylotype i tobacco strains in China

文献类型： 外文期刊

第一作者： Feng, Hui

作者： Feng, Hui;Wang, Xinwei;Wang, Jie;Wang, Ying;Yin, Li;Lu, Huaxin;Lu, Lihua

作者机构：

关键词： Ralstonia solanacearum; Homogenous electrochemical; Exo III -aided cyclic signal amplification; Plant bacterial wilt; Plant disease

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）

ISSN：

年卷期： 2024 年 403 卷

页码：

收录情况： SCI

摘要： Ralstonia solanacearum (R. solanacearum) bacterial wilt is a serious soil borne disease that has caused severe losses in tobacco production. In this study, a homogeneous electrochemical strategy based on the sequence of the RipTALI-9 gene is developed for the first time to sensitively detect R. solanacearum in tobacco. In virtue of the Exo III-aided cyclic signal amplification, the biosensor can sensitively monitor R. solanacearum gene, exhibiting the limit of detection (LOD) is as low as 9.53 ng center dot mL-1. The developed strategy completely has no need of DNA modification onto electrode surfaces and thus the detection shows high stability. Importantly, this biosensor also demonstrates excellent detection performance in real samples. In artificially inoculated tobacco samples, the LOD is 10.66 ng center dot mL-1, and in artificially inoculated soil sample, the detectable concentration is as low as 6.25 x 103 CFU center dot mL-1. These results show that the developed electrochemical assay has potential as an effective R. solanacearum diagnostic tool.

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