On-line pre-concentration and UV determination of DNA fragments by dynamic coating capillary electrophoresis and its application to detection of genetically modified oilseed rape based on PCR
文献类型: 外文期刊
第一作者: Chen, Hong
作者: Chen, Hong;Wu, Yu-Hua;Song, Dan-Yang;Zhang, Wen;Dong, Xu-Yan;Li, Pei-Wu;Lu, Chang-Ming
作者机构:
关键词: capillary electrophoresis;dynamic coating;DNA fragment;UV detection;transgenic rapeseed;genetically modified organisms;LASER-INDUCED FLUORESCENCE;POLYMERASE-CHAIN-REACTION;GEL-ELECTROPHORESIS;RESTRICTION FRAGMENTS;REACTION-PRODUCTS;ISOTACHOPHORETIC PRECONCENTRATION;MODIFIED MAIZE;FOOD ANALYSIS;SEPARATION;POLYACRYLAMIDE
期刊名称:MICROCHEMICAL JOURNAL ( 影响因子:4.821; 五年影响因子:4.364 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: In this work, it was demonstrated that on-line pre-concentration and separation of DNA fragments within bared silica column by dynamic coating capillary electrophoresis and UV detection. The DNA fragments were pre-concentrated with long electrokinetic injecting time (99 s), peak height increased dramatically as a function of injection time, especially for shorter length DNA. The concentration sensitivity of DNA fragments can be improved from 20- to 100-fold relative to a normal injection (5 s). The electro-osmotic flow (EOF) and DNA-wall interactions within the capillary were eliminated effectively by dynamic coating method. Employing 0.5% poly(ethylene oxide) (PEO) in Tris-phosphate-EDTA (TBE) buffer as sieving matrix, DNA fragments, ranging from 11 to 657 bp, were separated within 20 min. The linear coefficient of linear relation between the migration and DNA length is 0.999. The DNA fragments amplified from transgenic oilseed rape by polymerase chain reaction (PCR) were separated and detected by this method, demonstrating the potential use of this method for effective DNA analysis and detection of genetically modified organisms (GMO). (C) 2006 Elsevier B.V. All rights reserved.
分类号: O655
- 相关文献
作者其他论文 更多>>
-
Development of an enhanced hybrid attention YOLOv8s small object detection method for phenotypic analysis of root nodules
作者:Zhao, Ya;Zhang, Wen;Zhang, Liangxiao;Tang, Xiaoqian;Wang, Du;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Zhao, Ya;Li, Peiwu
关键词:Root nodules; YOLOv8s; Hybrid attention mechanism; Small object detection; Phenotypic characteristics
-
Generalization analysis of adversarial pairwise learning
作者:Wen, Wen;Li, Han;Wu, Lingjuan;Chen, Hong;Li, Han;Chen, Hong;Chen, Hong;Chen, Hong;Wu, Rui
关键词:Adversarial pairwise learning; Perturbation attacks; Error analysis; Generalization bounds
-
Metabolomics combined with biochemical analyses revealed phenolic profiles and antioxidant properties of rapeseeds
作者:Zhang, Yao;Lv, Xin;Wang, Dan;Zheng, Chang;Chen, Hong;Wei, Fang;Zhang, Yao;Yuan, Yongjun;Wei, Fang
关键词:Rapeseed; Phenolic compounds; Untargeted metabolomics; Molecular networking; Antioxidant activities
-
Partial replacement by ammonium nutrition enhances Brassica napus growth by promoting root development, photosynthesis, and nitrogen metabolism
作者:Zhang, Wen;Munyaneza, Venuste;Wang, Dandan;Huang, Chenfeng;Wu, Siyuan;Han, Mingcun;Ding, Guangda;Wang, Xu;Kant, Surya
关键词:Brassica napus; Ammonium; Nitrate; Photosynthesis; Nitrogen metabolism
-
Comprehensive physicochemical indicators analysis and quality evaluation model construction for the post-harvest ripening rapeseeds
作者:Xu, Qiuhui;Wang, Jie;Wang, Dan;Lv, Xin;Fu, Li;He, Ping;Mei, Desheng;Chen, Hong;Wei, Fang;Wei, Fang
关键词:Comprehensive evaluation model; Lipidomics; Physicochemical indicators; Post-harvest ripening; Quality improvement; Rapeseeds
-
Extensive cytonuclear discordance revealed by phylogenomic analyses suggests complex evolutionary history in the holly genus Ilex (Aquifoliaceae)
作者:Xu, Ke-Wang;Lin, Chen-Xue;Jiang, Lei;Guo, Zhong-Long;Li, Meng;Yang, Yi;Chen, Hong;Hao, Ming-Zhuo;Meng, Kai-Kai
关键词:Aquifoliales; Ilex; Incomplete lineage sorting; Hybridization; Phylogenomic discordance
-
Unravelling the gene regulatory network linking red leaf and red flesh traits in teinturier grape
作者:Li, Haoran;Zheng, Huan;Tao, Jianmin;Li, Haoran;Zhang, Yi;Sun, Chenxu;Huang, Liyuan;Dong, Yang;Yang, Yaxin;Li, Hui;Zheng, Huan;Tao, Jianmin;Zhang, Wen;Tao, Jianmin
关键词:Anthocyanins; Low-temperature; Grape leaf; Teinturier grape; Plant transformation