The Development of a Procedure for the Cryopreservation of the Callus of Anthurium andraeanum by Vitrification

文献类型： 外文期刊

第一作者： Zhang, Yiying

作者： Zhang, Yiying;Deng, Shan;Chu, Yunxia;Huang, Jingyan;Li, Shouguo;Ren, Li;Chen, Hairong;Lin, Huifeng;Lin, Fazhuang;Zhang, Sumei;Jiang, Weilan

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关键词： Anthurium andraeanum; callus culture; cryopreservation; vitrification

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 21 期

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收录情况： SCI

摘要： The cryopreservation of Anthurium andraeanum germplasm resources is extremely important for the production and selection of new varieties. At present, the cryopreservation procedure for the callus of A. andraeanum has not been established. In this study, the leaves of A. andraeanum were used as explants to culture the callus. The cryopreservation procedure of the callus by vitrification was initially established by using the orthogonal experimental method of four factors and three levels in the preculture, loading, and dehydration steps. Furthermore, the vitrification-based cryopreservation was optimized by changing the preculture temperature and loading solution and adding exogenous substances to the plant vitrification solution (PVS2). In this procedure, the callus was precultured at 25 degrees C for 2 d, and loaded in 50% PVS2 at 25 degrees C for 60 min. The callus was dehydrated with PVS2 containing 0.08 mM reduced glutathione (GSH) at 0 degrees C for 60 min. After rapid-cooling in liquid nitrogen for 1 h, it was rapid-warming in a water bath at 40 degrees C for 90 s and unloaded for 30 min. After 1 d of recovery, the cell relative survival rate of the cryopreserved callus was 64.60%. The results provide a valuable basic and effective method for the long-term conservation of A. andraeanum germplasm resources.

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