New insights on the evolution of nucleolar dominance in newly resynthesized hexaploid wheat Triticum zhukovskyi

文献类型： 外文期刊

第一作者： Huang, Yuhong

作者： Huang, Yuhong;Liu, Yang;Guo, Xianrui;Fan, Chaolan;Yi, Congyang;Shi, Qinghua;Liu, Chang;Yuan, Jing;Han, Fangpu;Huang, Yuhong;Yi, Congyang;Han, Fangpu;Su, Handong;Liu, Dengcai;Yang, Wuyun

作者机构：

关键词： nucleolus organizer region; ribosomal DNA genes; nucleolar dominance; epigenetics; Triticum zhukovskyi

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Nucleolar dominance (ND) is a widespread epigenetic phenomenon in hybridizations where nucleolus transcription fails at the nucleolus organizer region (NOR). However, the dynamics of NORs during the formation of Triticum zhukovskyi (GGA(u)A(u)A(m)A(m)), another evolutionary branch of allohexaploid wheat, remains poorly understood. Here, we elucidated genetic and epigenetic changes occurring at the NOR loci within the A(m), G, and D subgenomes during allopolyploidization by synthesizing hexaploid wheat GGA(u)A(u)A(m)A(m) and GGA(u)A(u)DD. In T. zhukovskyi, A(u) genome NORs from T. timopheevii (GGA(u)A(u)) were lost, while the second incoming NORs from T. monococcum (A(m) A(m)) were retained. Analysis of the synthesized T. zhukovskyi revealed that rRNA genes from the A(m) genome were silenced in F-1 hybrids (GA(u)A(m)) and remained inactive after genome doubling and subsequent self-pollinations. We observed increased DNA methylation accompanying the inactivation of NORs in the A(m) genome and found that silencing of NORs in the S-1 generation could be reversed by a cytidine methylase inhibitor. Our findings provide insights into the ND process during the evolutionary period of T. zhukovskyi and highlight that inactive rDNA units may serve as a 'first reserve' in the form of R-loops, contributing to the successful evolution of T. zhukovskyi.

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