Cellular vimentin regulates the infectivity of Newcastle disease virus through targeting of the HN protein

文献类型： 外文期刊

第一作者： Lu, Xiaolong

作者： Lu, Xiaolong;Liu, Kaituo;Chen, Yu;Gao, Ruyi;Hu, Zenglei;Hu, Jiao;Gu, Min;Hu, Shunlin;Jiao, Xinan;Wang, Xiaoquan;Liu, Xiufan;Liu, Xiaowen;Hu, Zenglei;Hu, Jiao;Gu, Min;Hu, Shunlin;Jiao, Xinan;Wang, Xiaoquan;Liu, Xiufan;Liu, Xiaowen;Ding, Chan;Jiao, Xinan;Liu, Xiufan;Hu, Zenglei;Jiao, Xinan;Ding, Chan;Jiao, Xinan

作者机构： Yangzhou Univ, Coll Vet Med, Anim Infect Dis Lab, 48 East Wenhui Rd, Yangzhou 225009, Jiangsu, Peoples R China;Yangzhou Univ, Jiangsu Key Lab Zoonosis, Yangzhou 225009, Peoples R China;Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Peoples R China;Yangzhou Univ, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ China, Yangzhou 225009, Peoples R China;Chinese Acad Agr Sci, Shanghai Vet Res Inst, Dept Avian Infect Dis, Shanghai 200000, Peoples R China

关键词： Newcastle disease virus; HN; vimentin; viral infection; macrophage

期刊名称：VETERINARY RESEARCH （ 2022影响因子：4.4； 五年影响因子：4.3 ）

ISSN： 0928-4249

年卷期： 2023 年 54 卷 1 期

页码：

收录情况： SCI

摘要： The haemagglutinin-neuraminidase (HN) protein plays a crucial role in the infectivity and virulence of Newcastle disease virus (NDV). In a previous study, the mutant HN protein was identified as a crucial virulence factor for the velogenic variant NDV strain JS/7/05/Ch, which evolved from the prototypic vaccine strain Mukteswar. Furthermore, macrophages are the main susceptible target cells of NDV. However, the possible involvement of cellular molecules in viral infectivity remains unclear. Herein, we elucidate the crucial role of vimentin, an intermediate filament protein, in regulating NDV infectivity through targeting of the HN protein. Using LC-MS/MS mass spectrometry and coimmunoprecipitation assays, we identified vimentin as a host protein that differentially interacted with prototypic and mutant HN proteins. Further analysis revealed that the variant NDV strain induced more significant rearrangement of vimentin fibres compared to the prototypic NDV strain and showed an interdependence between vimentin rearrangement and virus replication. Notably, these mutual influences were pronounced in HD11 chicken macrophages. Moreover, vimentin was required for multiple infection processes of the variant NDV strain in HD11 cells, including viral internalization, fusion, and release, while it was not necessary for those of the prototypic NDV strain. Collectively, these findings underscore the pivotal role of vimentin in NDV infection through targeting of the HN protein, providing novel targets for antiviral treatment strategies for NDV.

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