Research Progress in Isotope Labeling/Tags-Based Protein Quantification and Metrology Technologies
文献类型： 外文期刊
第一作者： Wu, Fan
作者： Wu, Fan;Chen, Rui;Chu, Zhanying;Zhai, Rui;Wu, Fan;Zhang, Chenhuan;Han, Bin
作者机构：
关键词： mass spectrometry; protein quantification; stable isotope labeling; isobaric tagging; proteinmetrology
期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：3.6； 五年影响因子：4.0 ）
ISSN： 1535-3893
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： Advanced liquid chromatogram-mass spectrometry (LC-MS) and automated large-scale data processing have made MS-based quantitative analysis increasingly useful for research in fields such as biology, medicine, food safety, and beyond. This is because MS-based quantitative analysis can accurately and sensitively analyze thousands of proteins and peptides in a single experiment. However, the precision, coverage, complexity, and resilience of conventional quantification methods vary as a result of the modifications to the analytic environment and the physicochemical characteristics of analytes. Therefore, specially designed approaches are necessary for sample preparation. Dozens of methods have been developed and adapted for these needs based on stable isotopic labeling or isobaric tagging, each with distinct characteristics. In this review, we will summarize the leading strategies and techniques used thus far for MS-based protein quantification as well as analyze the advantages and shortcomings of different approaches. Additionally, we provide an overview of protein metrology development.
分类号：
- 相关文献
作者其他论文 更多>>
-
Explore the antioxidative and tyrosinase inhibitory activity of green sericin resources from different fibroin-deficient silkworm varieties
作者：Cao, Xinyu;Ding, Xinyi;Chen, Rui;Li, Yurong;Tehoungue, Akoumay;Kang, Guoping;Zhang, Guozheng;Zhang, Yeshun;Li, Yurong;Kang, Guoping;Zhang, Guozheng;Zhang, Yeshun;Huang, Wengong;Kang, Guoping;Zhang, Yeshun
关键词：Sericin; Silk fibroin-deficient resources; Antioxidative activity; Tyrosinase inhibitory activity
-
A particle counting immunosensor for the sensitive detection of ochratoxin A via click chemistry-mediated signal amplification
作者：Chen, Rui;Li, Shuhuai;Ma, Xionghui;Pang, Chaohai;Dong, Yongzhen;Chen, Yiping;Wang, Shu;Zhang, Jiangjiang
关键词：SiO 2 @CuO 2 nanoparticles; Particle counter; Polystyrene microspheres; Click chemistry; Ochratoxin A
-
Editing the RR-TZF Gene Subfamily in Rice Uncovers Potential Risks of CRISPR/Cas9 for Targeted Genetic Modification
作者：Zhou, Shufen;Lu, Xiaozhuan;Wu, Weiren;Zhou, Shufen;Tian, Dagang;Liu, Huaqing;Zhang, Di;Chen, Rui;Yang, Shaohua;Wang, Feng
关键词：CRISPR/Cas9;
RR-TZF gene subfamily; rice (Oryza sativa L.); off-target effects; inheritable mutagenicity -
Genome-wide identification of the cotton FAR gene family reveals GhFAR3 as a positive regulator of Verticillium dahliae resistance
作者：Cheng, Wenhan;Chen, Rui;Gao, Siqi;Xiong, Xianpeng;He, Zhaojie;Aini, Nurimanguli;Zhao, Zengqiang;Wang, Ning;Feng, Keyun
关键词：Cotton; Fatty acyl-CoA reductase; FAR3;
Verticillium dahliae ; Suberin -
The Effect of the Fresh Latex Ratio on the Processing and Dynamic Properties of Bio-Coagulated Natural Rubber
作者：Li, Jianwei;Ding, Li;Huang, Honghai;Dai, Tuo;Zhao, Liguang;Wu, Fan;Gui, Hongxing;Li, Yun;Xu, Yingguang
关键词：variety; fresh latex; ratio; processing property; physical and mechanical property
-
Phenolic Acid and Flavonoid Content Analysis with Antioxidant Activity Assessment in Chinese C. pi. Shen Honey
作者：Qi, Ningxin;Zhao, Wen;Xue, Chenghua;Zhang, Lin;Hu, Han;Jin, Yue;Xue, Xiaofeng;Chen, Rui;Zhang, Jinzhen
关键词：phenolic compound; antioxidant activities;
C. pi. Shen honey; different botanic origins -
Infectious bursal disease virus affecting interferon regulatory factor 7 signaling through VP3 protein to facilitate viral replication
作者：Wang, Zhiyuan;Chen, Yang;Chen, Yanyan;Chen, Rui;Hu, Shichen;Li, Yihai;He, Xiumiao;Wang, Weiwei;Wei, Ping;Chen, Hongjun
关键词：infectious bursal disease virus (IBDV); interferon regulatory factor 7 (IRF7); antiviral response; IBDV VP3 protein; viral replication
