A cytosolic thiouridylase gene MoCTU2 in Magnaporthe oryzae is important for vegetative hyphal growth, conidiation, and responses to rapamycin and high temperature

文献类型： 外文期刊

第一作者： Yan, Han

作者： Yan, Han;Chen, Lijie;Yan, Han;Zuo, Yushan;Zhang, Huixia;Liu, Cuicui;Yang, Jun

作者机构：

关键词： Rice blast fungus; Pyricularia oryzae; TOR; tRNA modification; Thiolation

期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.741； 五年影响因子：2.576 ）

ISSN： 0885-5765

年卷期： 2022 年 121 卷

页码：

收录情况： SCI

摘要： Cytoplasmic thiouridylase Ctu1-Ctu2 protein complex is responsible for thiolation on the wobble base U34 of tRNAs and plays an important role in cell development in model organisms. However, the biological function of this protein complex in plant pathogenic fungi remains unknown. In this study, we identified a cytosolic thiouridylase gene MoCTU2 in the rice blast fungus Magnaporthe oryzae. The delta Moctu2 mutant showed reduced vegetative hyphal growth and conidiation, and increased responses to rapamycin and high temperature. How-ever, MoCTU2 had a minor impact on the ability of the mutant causing blast disease on rice. MoCTU2 was constitutively expressed at various developmental stages, with a peak during appressorium formation; the MoCtu2-GFP fusion was evenly distributed in the cytoplasm. Altogether, our findings indicate that the cytosolic thiouridylase gene MoCTU2 is required for fungal development, conidiation, and responses to rapamycin and high temperature in the blast fungus.

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