Individual and combined effects of salinity and waterlogging on Cry1Ac expression and insecticidal efficacy of Bt cotton

文献类型： 外文期刊

第一作者： Luo, Zhen

作者： Luo, Zhen;Dong, Hezhong;Li, Weijiang;Ming, Zhao;Zhu, Yuqing

作者机构：

关键词： salinity;waterlogging;sodium chloride stress

期刊名称：CROP PROTECTION （ 影响因子：2.571； 五年影响因子：3.11 ）

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年卷期：

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收录情况： SCI

摘要： Salinity, waterlogging and a combination of both stresses are severe threats to plant growth, development and yield of field-grown cotton (Gossypium hirsutum L), but their individual or combined effects on insecticidal efficacy of Bacillus thuringiensis (Bt) transgenic cotton and the underlying mechanisms are not well understood. In the present study, two cotton cultivars (33B and SCRC17) containing the Cry1Ac insecticidal protein gene were planted in 10 L pots filled with soil and allowed to grow in a greenhouse. The potted plants were either treated with NaCl (5 mg/g, w/w), waterlogging, or a combination of both stresses at the three true-leaf stage, and levels of total soluble protein. Bt insecticidal protein, gossypol and the control efficacy as indicated by mortality of bollworm larvae were examined at 7-day intervals after stress. Waterlogging and a combination of salinity and waterlogging reduced total protein content by 40-46% and 45-65% and Bt protein content by 38-50% and 45-72% from 7 to 21 days after stress, relative to the non-stressed control, respectively. The control efficacy was significantly reduced by either waterlogging or the combined stress. Regression analysis indicated that Bt protein content was correlated to total soluble protein content (R-2 = 0.7677*), while et cotton efficacy was correlated to Bt protein level (R-2 = 0.7917**). Salinity reduced Bt protein by 11-22% and total soluble protein by 5.7-7.2% from 7 to 21 days after NaCl stress, but did not result in reduction in control efficacy. It is concluded that reduced bollworm control efficacy under waterlogging or the combined stress could be mainly attributed to the declined levels of Bt protein, which is closely associated with the inhibited nitrogen metabolism by stresses. As one of the secondary compounds that are toxic to pests, increases in gossypol may be involved in maintaining the efficacy when Bt protein level was reduced under salinity.

分类号： S5

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