GENE CLONING OF BOTRYTIS CINEREA MRR1 AND DETECTION OF ITS RESISTANCE TO SIX SOIL POLLUTANTS FOR ENVIRONMENTAL PROTECTION ENQUIREMENT

文献类型： 外文期刊

第一作者： Shen, Fengying

作者： Shen, Fengying;Wang, Jiao;Li, Yaning;Liu, Daqun;Shen, Fengying;Wu, Weigang;Han, Xing;Liu, Daqun;Zhang, Yanjie

作者机构：

关键词： acinerea; mrr1 gene; multiple site mutation; soil pollutant; resistance test

期刊名称：FRESENIUS ENVIRONMENTAL BULLETIN （ 影响因子：0.489； 五年影响因子：0.479 ）

ISSN： 1018-4619

年卷期： 2021 年 30 卷 6B 期

页码：

收录情况： SCI

摘要： In this paper, the mrr1 gene of Botrytis cinerea (B.cinerea)was cloned, and its resistance to six types of soil pollutants was tested, The plate transparent circle method was used to screen strains, and a strain of B.cinerea PTH-mrr1 with activity against multiple soil pollutants was obtained. Real-Time PCR found that mrr1 gene was significantly down-regulated in this strain, PCR technology was used to successfully clone the mrr1 mutant gene from this strain, Sequencing results revealed that the mrr1 mutant gene contained 2 490 bases and 830 amino acids, Compared with the normal base sequence, multiple base insertions or deletions occurred at multiple sites. The mrr1 mutant gene was expressed in E.coli to obtain a prokaryotic expression product, which was then used to act on a variety of soil pollutants, It was found that E. coil with mrr1 gene mutation was resistant to carbendazim, procymidone, iprodione, pyrimethanil and thiram. The prokaryotic expression conditions were further optimized, and it was found that when E. coil containing mrr1 gene was cultured with shaking at 30 degrees C for 24 hours, and the IPTG concentration was 0,75 mmol/L, the expression level of mini protein was the highest. Furthermore, it has the hest resistance to carbendazim, procymidone, iprodione, pyrimethanil and thiram. In conclusion, in this study, we discussed the gene function of B. cinerea mrrl and determined its resistance to various soil pollutants. This research laid a theoretical foundation for the future development of antisoi I pollutant products,

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