Mechanism Analysis of UCP2 During the Oxidative Stress Injury of Intestinal Porcine Epithelial Cell Line-J2

文献类型： 外文期刊

第一作者： Su, Weide

作者： Su, Weide;Xu, Chuanhui;Jiang, Hongping;Song, Wenjing;Xiong, Pingwen;Chen, Jiang;Ai, Gaoxiang;Song, Qiongli;Zou, Zhiheng;Wei, Qipeng;Chen, Xiaolian;Su, Weide;Xu, Chuanhui;Jiang, Hongping;Song, Wenjing;Xiong, Pingwen;Chen, Jiang;Ai, Gaoxiang;Song, Qiongli;Zou, Zhiheng;Wei, Qipeng;Chen, Xiaolian

作者机构：

关键词： UCP2; oxidative stress; porcine intestinal epithelial cells; genipin; apoptosis

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2025 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Oxidative stress poses a significant challenge in livestock production, impairing intestinal function, nutrient absorption, and overall animal performance. Uncoupling protein 2 (UCP2) is a mitochondrial regulator known for its protective effects against oxidative damage, but its specific function in porcine intestinal epithelial cells and its regulation by genipin-a natural UCP2 inhibitor with potential therapeutic properties-remains unclear. In this study, we cloned and overexpressed the porcine UCP2 gene in intestinal porcine epithelial cells (IPEC-J2), generating a stable UCP2-overexpressing cell line (IPEC-J2-UCP2). Under hydrogen peroxide-induced oxidative stress, UCP2 overexpression significantly improved cell viability, reduced reactive oxygen species (ROS) levels, and enhanced antioxidant enzyme activities (SOD, GPx, and CAT). Additionally, UCP2 upregulated the anti-apoptotic gene Bcl-2 and downregulated pro-apoptotic genes (Fas, Caspase-3, and Bax), indicating a protective role against oxidative stress-induced apoptosis. We also investigated the regulatory effects of genipin on UCP2. Under non-stress conditions, genipin mildly promoted anti-apoptotic gene expression. However, under oxidative stress, genipin strongly inhibited UCP2 expression, exacerbated ROS accumulation, reduced cell viability, and increased expression of pro-apoptotic markers, particularly Caspase-3 and Bax. These findings reveal that UCP2 plays a critical role in protecting porcine intestinal epithelial cells from oxidative injury and that genipin exerts context-dependent effects on cell fate by modulating UCP2. This study provides a mechanistic basis for targeting UCP2 to manage oxidative stress and improve intestinal health and performance in pigs.

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