Studying the role of Bombyx mori molybdenum cofactor sulfurase in Bombyx mori nucleopolyhedrovirus infection

文献类型： 外文期刊

第一作者： Lv, Jun-li

作者： Lv, Jun-li;Lai, Wen-qing;Gong, Yu-quan;Zheng, Kai-yi;Zhang, Xiao-ying;Wang, Xue-yang;Li, Mu-wang;Wang, Xue-yang;Li, Mu-wang;Dai, Li-shang;Wang, Xue-yang;Li, Mu-wang

作者机构： Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang, Jiangsu, Peoples R China;Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr, Zhenjiang, Jiangsu, Peoples R China;Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 212100, Zhejiang, Peoples R China;Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212100, Jiangsu, Peoples R China

关键词： BmMoCoS; BmNPV; Bombyx mori; response mechanism; uric acid pathway

期刊名称：INSECT MOLECULAR BIOLOGY （ 2022影响因子：2.6； 五年影响因子：2.9 ）

ISSN： 0962-1075

年卷期： 2024 年 33 卷 3 期

页码：

收录情况： SCI

摘要： Molybdenum cofactor sulfurase (MoCoS) is a key gene involved in the uric acid metabolic pathway that activates xanthine dehydrogenase to synthesise uric acid. Uric acid is harmful to mammals but plays crucial roles in insects, one of which is the immune responses. However, the function of Bombyx mori MoCoS in response to BmNPV remains unclear. In this study, BmMoCoS was found to be relatively highly expressed in embryonic development, gonads and the Malpighian tubules. In addition, the expression levels of BmMoCoS were significantly upregulated in three silkworm strains with different levels of resistance after virus infection, suggesting a close link between them. Furthermore, RNAi and overexpression studies showed that BmMoCoS was involved in resistance to BmNPV infection, and its antivirus effects were found to be related to the regulation of uric acid metabolism, which was uncovered by inosine- and febuxostat-coupled RNAi and overexpression. Finally, the BmMoCoS-mediated uric acid pathway was preliminarily confirmed to be a potential target to protect silkworms from BmNPV infection. Overall, this study provides new evidence for elucidating the molecular mechanism of silkworms in response to BmNPV infection and new strategies for the prevention of viral infections in sericulture.

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