Isolation and Characterization of Mlo and NBS-LRR-like Gene Sequences in Wheat

文献类型： 外文期刊

第一作者： Li, AL

作者： Li, AL;Kong, XY;Zhou, RH;Ma, ZY;Jia, JZ

作者机构：

关键词： Mlo;NBS-LRR;cDNA cloning;NIL;RGA

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

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年卷期：

页码：

收录情况： SCI

摘要： Based on both cDNA sequence of barley powdery mildew resistance control element Mlo and DNA sequence of the known putative disease resistance gene from Triticum monococcum L. , we designed some primers to amplify resistant homologous sequences in the near isogenic lines (NILs) of powdery mildew resistance using RT-PCR method. Two expressed cDNA fragments were isolated from wheat genome. One showed 83% homology to the Mlo gene of barley. The other contained two possible open reading frames (ORFs). NBS conservative domains 2, 3 of disease resistance gene and 13 LRR structures similar to rice Pib protein terminal were found respectively in the two ORFs. It indicated that the latter fragment belongs to NBS-LRR-like genes. The obvious difference of RT-PCR products was observed between the before challenged and the challenged for 72 h by Blumeria graminis f. sp. tritici, which implied that this sequence could be associated with disease resistance of wheat. Using nulli-tetrasomic lines of "Chinese Spring", the NBS-LRR-like gene had been located on chromosome 1D.

分类号： Q94

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