Surfactins and Iturins Produced by Bacillus velezensis Jt84 Strain Synergistically Contribute to the Biocontrol of Rice False Smut

文献类型： 外文期刊

第一作者： Zhang, Rongsheng

作者： Zhang, Rongsheng;Yu, Junjie;Yang, Lin;Qiao, Junqing;Qi, Zhongqiang;Yu, Mina;Du, Yan;Song, Tianqiao;Cao, Huijuan;Pan, Xiayan;Liu, Youzhou;Liu, Yongfeng

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关键词： Bacillus velezensis; surfactins; iturins; Ustilaginoidea virens; rice false smut

期刊名称：AGRONOMY-BASEL （ 影响因子：3.4； 五年影响因子：3.8 ）

ISSN：

年卷期： 2024 年 14 卷 10 期

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收录情况： SCI

摘要： Rice false smut, caused by the plant pathogenic fungus Ustilaginoidea virens, is widespread in rice-growing regions globally, severely compromising rice quality and production. Employing Bacillus spp. to control rice false smut represents an effective and environmentally sustainable strategy for disease management. The lipopeptides produced by Bacillus velezensis Jt84 demonstrated robust inhibitory effects against U. virens, resulting in abnormal mycelial morphology and spore germination. Iturins were identified as essential for the antifungal activity against U. virens, as confirmed by mutagenesis experiments that suppressed iturin biosynthesis. The surfactin-deficient mutant exhibited inhibitory effects against U. virens comparable to the wild-type, indicating that the absence of surfactins did not diminish its antifungal activity. Both the Jt84 triangle srf and Jt84 triangle itu mutants displayed reduced biofilm formation capabilities compared to the wild-type, with the Jt84 triangle srf mutant being particularly impaired and unable to form a complete biofilm. Regarding swarming motility, the triangle srf mutant exhibited a significant reduction compared to the wild-type, whereas the Jt84 triangle itu mutant showed a modest increase. Colonization experiments revealed that the Jt84 triangle srf mutant strain had significantly lower colonization on rice leaf surfaces than the wild-type strain, highlighting the critical role of surfactins in the colonization of B. velezensis Jt84 on rice leaves. In conclusion, our research demonstrated that surfactins and iturins have distinct functionalities and act synergistically to contribute to the biocontrol of rice false smut in B. velezensis Jt84. This synergy is achieved through their potent antifungal effects, biofilm formation, and successful colonization.

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