Sleep deprivation boosts O2•- levels in the brains of mice as visualized by a Golgi apparatus-targeted ratiometric fluorescence nanosensor

文献类型： 外文期刊

第一作者： Song, Wei

作者： Song, Wei;Yao, Chunxia;Lu, Yangyang;Qian, Qunli;Song, Weiguo;Wu, Jun;Shi, Wenru;Li, Huiru;Huang, Hong;Wang, Weikang

作者机构：

关键词： Sleep deprivation; Golgi apparatus; Mouse brain; Carbon nanodots; Polymer dots

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.7； 五年影响因子：5.4 ）

ISSN： 0026-3672

年卷期： 2024 年 191 卷 5 期

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收录情况： SCI

摘要： Sleep deprivation (SD) is highly prevalent in the modern technological world. Emerging evidence shows that sleep deprivation is associated with oxidative stress. At the organelle level, the Golgi apparatus actively participates in the stress response. In this study, to determine whether SD and Golgi apparatus stress are correlated, we rationally designed and fabricated a novel Golgi apparatus-targeted ratiometric nanoprobe called Golgi dots for O-2(center dot-) detection. This probe exhibits high sensitivity and selectivity in cells and brain slices of sleep-deprived mice. Golgi dots can be readily synthesized by coprecipitation of Golgi-F127, an amphiphilic polymer F127 modified with a Golgi apparatus targeting moiety, caffeic acid (CA), the responsive unit for O-2(center dot-), and red emissive carbon nanodots (CDs), which act as the reference signal. The fluorescence emission spectrum of the developed nanoprobe showed an intense peak at 674 nm, accompanied by a shoulder peak at 485 nm. As O-2(center dot-) was gradually added, the fluorescence at 485 nm continuously increased; in contrast, the emission intensity at 674 nm assigned to the CDs remained constant, resulting in the ratiometric sensing of O-2(center dot-). The present ratiometric nanoprobe showed high selectivity for O-2(center dot-) monitoring due to the specific recognition of O-2(center dot-) by CA. Moreover, the Golgi dots exhibited good linearity with respect to the O-2(center dot-) concentration within 5 to 40 mu M, and the limit of detection (LOD) was similar to 0.13 mu M. Additionally, the Golgi dots showed low cytotoxicity and an ability to target the Golgi apparatus. Inspired by these excellent properties, we then applied the Golgi dots to successfully monitor exogenous and endogenous O-2(center dot-) levels within the Golgi apparatus. Importantly, with the help of Golgi dots, we determined that SD substantially elevated O-2(center dot-) levels in the brain.

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