Novel ACE inhibitory peptides derived from bighead carp (Aristichthys nobilis) hydrolysates: Screening, inhibition mechanisms and the bioconjugation effect with graphene oxide

文献类型： 外文期刊

第一作者： Chen, Mengting

作者： Chen, Mengting;Wang, Lan;Zheng, Changliang;Xiong, Guangquan;Shi, Liu;Chen, Sheng;Wu, Wenjin;Chen, Mengting;Ma, Aimin;Hu, Kai;Xiang, Aoli;Sun, Zhida;Xie, Bijun

作者机构：

关键词： ACE inhibitory activity; Bioactive peptides; Molecular docking; Graphene oxide; Bioconjugation

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.2； 五年影响因子：5.4 ）

ISSN： 2212-4292

年卷期： 2023 年 52 卷

页码：

收录情况： SCI

摘要： The ACE inhibitory peptides have been characterized from the enzymatic digestion product of bighead carp protein and bioconjugated with graphene oxide (GO) to enhance its activity. The results showed that aspartic acid and glutamic acid had the highest levels in ultrafiltration fractions (<5 kDa), where eight potential ACE inhibitory peptides were also identified (ADSNHKAF, KLWHHTF, LLRLHF, PPSEPTKL, VEKFPLF, YLRLHF, YYKLKPLL, YYKLKPML). Among the eight peptides, YLRLHF showed the best ACE inhibitory activity (IC50 = 121.90 mu M) and was a competitive inhibitor. Molecular docking experiments showed that YLRLHF formed four hydrogen bond interactions in the ACE protein pocket, coordination bonding with Zn2+, and 7C-7C conjugation interactions to His421. GO elevated the ACE inhibitory activity of YLRLHF (at 0.1 mg/mL) from 43.36% to 51.72%. The structural characterization results obtained from FI-IR, XPS, SEM, and TEM demonstrated the successful combination of GO and YLRLHF. Additionally, biocouples of ACE inhibitory peptides from bighead carp proteins with GO might be potential candidates for future functional foods and antihypertensive drugs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序