Molecular modification and biotechnological applications of microbial aspartic proteases

文献类型： 外文期刊

第一作者： Herman, Richard Ansah

作者： Herman, Richard Ansah;Zhang, Wen-Xin;Li, Zong-Nan;Zhu, Xuan;Ackah, Michael;Yuan, Shuang-Shuang;You, Shuai;Wang, Jun;Herman, Richard Ansah;Ayepa, Ellen;You, Shuai;Wang, Jun

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关键词： Aspartic proteases; molecular modification; biocatalysis; biotechnological applications; microbial engineering

期刊名称：CRITICAL REVIEWS IN BIOTECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）

ISSN： 0738-8551

年卷期： 2023 年

页码：

收录情况： SCI

摘要： The growing preference for incorporating microbial aspartic proteases in industries is due to their high catalytic function and high degree of substrate selectivity. These properties, however, are attributable to molecular alterations in their structure and a variety of other characteristics. Molecular tools, functional genomics, and genome editing technologies coupled with other biotechnological approaches have aided in improving the potential of industrially important microbial proteases by addressing some of their major limitations, such as: low catalytic efficiency, low conversion rates, low thermostability, and less enzyme yield. However, the native folding within their full domain is dependent on a surrounding structure which challenges their functionality in substrate conversion, mainly due to their mutual interactions in the context of complex systems. Hence, manipulating their structure and controlling their expression systems could potentially produce enzymes with high selectivity and catalytic functions. The proteins produced by microbial aspartic proteases are industrially capable and far-reaching in regulating certain harmful distinctive industrial processes and the benefits of being eco-friendly. This review provides: an update on current trends and gaps in microbial protease biotechnology, exploring the relevant recombinant strategies and molecular technologies widely used in expression platforms for engineering microbial aspartic proteases, as well as their potential industrial and biotechnological applications.

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