A New Isolated Fungus and Its Pathogenicity for Apis mellifera Brood in China

文献类型： 外文期刊

第一作者： Aynalem, Tessema

作者： Aynalem, Tessema;Meng, Lifeng;Getachew, Awraris;Wu, Jiangli;Yu, Huimin;Tan, Jing;Li, Nannan;Xu, Shufa;Aynalem, Tessema;Getachew, Awraris

作者机构： Chinese Acad Agr Sci, State Key Lab Resource Insects, Inst Apicultural Res, Beijing 100093, Peoples R China;Bahir Dar Univ, Coll Agr & Environm Sci, POB 26, Bahir Dar, Ethiopia

关键词： honeybee; larvae; fungi; Rhizopus oryzae; pathogenicity

期刊名称：MICROORGANISMS （ 2022影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 12 卷 2 期

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收录情况： SCI

摘要： In this article, we report the pathogenicity of a new strain of fungus, Rhizopus oryzae to honeybee larvae, isolated from the chalkbrood-diseased mummies of honeybee larvae and pupae collected from apiaries in China. Based on morphological observation and internal transcribed spacer (ITS) region analyses, the isolated pathogenic fungus was identified as R. oryzae. Koch's postulates were performed to determine the cause-and-effect pathogenicity of this isolate fungus. The in vitro pathogenicity of this virulent fungus in honeybees was tested by artificially inoculating worker larvae in the lab. The pathogenicity of this new fungus for honeybee larvae was both conidial-concentration and exposure-time dependent; its highly infectious and virulent effect against the larvae was observed at 1 x 10(5) conidia/larva in vitro after 96 h of challenge. Using probit regression analysis, the LT50 value against the larvae was 26.8 h at a conidial concentration of 1 x 10(5) conidia/larva, and the LC50 was 6.2 x 10(3) conidia/larva. These results indicate that the new isolate of R. oryzae has considerable pathogenicity in honeybee larvae. Additionally, this report suggests that pathogenic phytofungi may harm their associated pollinators. We recommend further research to quantify the levels, mechanisms, and pathways of the pathogenicity of this novel isolated pathogen for honeybee larvae at the colony level.

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