The inhibitory effect of swine TAB1 on the replication of pseudorabies virus

文献类型： 外文期刊

第一作者： Xia, Xiujuan

作者： Xia, Xiujuan;Wu, Xiangju;Jiang, Dandan;Hu, Yue;Cong, Xiaoyan;Li, Juntong;Du, Yijun;Qi, Jing;Xia, Xiujuan;Dai, Meixue;Du, Yijun;Qi, Jing

作者机构：

关键词： STAB1; Pseudorabies virus; Inflammatory factors; Chemokines; IFN-beta

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.4； 五年影响因子：2.6 ）

ISSN： 0378-1135

年卷期： 2024 年 296 卷

页码：

收录情况： SCI

摘要： TAK1-binding protein 1 (TAB1) assembles with TAK1 through its C-terminal domain, leading to the selfphosphorylation and activation of TAK1, which plays an important role in the activation of NF- kappa B and MAPK signaling pathway. Pseudorabies virus (PRV) is the pathogen of Pseudorabies (PR), which belongs to the Alphaherpesvirus subfamily and causes serious economic losses to the global pig industry. However, the impact of swine TAB1 (sTAB1) on PRV infection has not been reported. In this study, evidence from virus DNA copies, virus titer and western blotting confirmed that sTAB1 could inhibit PRV replication and knockout of sTAB1 by CRISPRCas9 gene editing system could promote PRV replication. Further mechanistic studies by real-time PCR and luciferase reporter gene assay demonstrated that sTAB1 could enhance the production of inflammatory factors and chemokines, IFN- beta transcription level and IFN- beta promoter activity after PRV infection. In summary, we clarify the underlying mechanism of sTAB1 in inhibiting PRV replication for the first time, which provides a new idea for preventing PRV infection and lays a foundation for PRV vaccine development.

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