Genome-Wide Association Study (GWAS) Identifies Key Candidate Genes Associated with Leaf Size in Alfalfa (Medicago sativa L.)

文献类型： 外文期刊

第一作者： Xu, Ming

作者： Xu, Ming;Jiang, Xueqian;He, Fei;Sod, Bilig;Zhang, Fan;Long, Ruicai;Li, Mingna;Wang, Xue;Yang, Qingchuan;Kang, Junmei;Xu, Ming;Cong, Lili;Yang, Tianhui;Yang, Tianhui;Zhang, Tiejun

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关键词： alfalfa; GWAS; leaf size

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.6； 五年影响因子：3.6 ）

ISSN：

年卷期： 2023 年 13 卷 12 期

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收录情况： SCI

摘要： Leaf size significantly impacts photosynthetic capacity and forage yield in alfalfa, a major legume forage crop. Therefore, elucidating the genetic factors governing leaf development is critical for breeding improved alfalfa varieties. In this study, a genome-wide association analysis (GWAS) was performed to dissect the genetic architecture of leaf length (LL) and leaf width (LW) using 220 alfalfa accessions phenotyped over three years. Substantial variation for both traits was observed across environments, with coefficients of variation ranging from 10.09-16.53%. GWAS identified 26 significant SNPs associated with leaf morphology spread across seven chromosomes. Each SNP accounts for 9.7-15.6% of the phenotypic variance. Haplotype analyses confirmed positive correlations between the number of superior alleles and both LL and LW. BLAST searches revealed six candidate genes involved in leaf development within 20 kb flanking regions of significant SNPs. Our results provide novel marker-trait associations and candidate loci to facilitate molecular breeding efforts to optimize leaf size and improve productivity in alfalfa. This study establishes a foundation for integrating favorable alleles into future alfalfa varieties.

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