文献类型： 外文期刊
第一作者： Ma, Guigen
作者： Ma, Guigen;Kuang, Yongjie;Lu, Zhenwan;Li, Xueqi;Xu, Ziyan;Ren, Bin;Zhou, Xueping;Zhou, Huanbin;Ma, Guigen;Kuang, Yongjie;Lu, Zhenwan;Li, Xueqi;Xu, Ziyan;Ren, Bin;Zhou, Huanbin;Zhou, Xueping
作者机构：
关键词： base editing; genome editing; rice; Sc++
期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）
ISSN： 1672-9072
年卷期： 2021 年 63 卷 9 期
页码：
收录情况： SCI
摘要： Streptococcus canis Cas9 (ScCas9) is an RNA-guided endonuclease with NNG protospacer adjacent motif (PAM) specificity whose genome-editing activity in rice is locus-dependent. Here we investigated the performance of a ScCas9 variant named Sc++ at different NNG PAM sites in the rice genome; Sc++ harbors a T1227K mutation and the substitution of a positively charged loop (residues 367-376). Sc++ nuclease achieved broader genome editing compared to the original ScCas9, and its nickase improved targeted base editing in transgenic rice plants. Using the high-efficiency adenine base editor rBE73b, we generated many new OsGS1 alleles suitable for screening of rice germplasm for potential herbicide resistance in the future. The CRISPR/Sc++ system expands the genome-editing toolkit for rice.
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