Regulation by distinct MYB transcription factors defines the roles of OsCYP86A9 in anther development and root suberin deposition

文献类型： 外文期刊

第一作者： Huang, Xiaoyan

作者： Huang, Xiaoyan;Li, Yiqi;Chang, Zhenyi;Yan, Wei;Wang, Changjian;Zheng, Minting;Li, Zhiai;Tang, Xiaoyan;Wu, Jianxin;Xu, Chunjue;Tang, Xiaoyan;Xu, Chunjue;Tang, Xiaoyan;Zhang, Baolei;Xia, Jixing;He, Zhaohuan;Li, Guoliang

作者机构：

关键词： Oryza sativa; OsMYBs; anther development; suberin

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Cytochrome P450 proteins (CYPs) play critical roles in plant development and adaptation to fluctuating environments. Previous reports have shown that CYP86A proteins are involved in the biosynthesis of suberin and cutin in Arabidopsis. However, the functions of these proteins in rice remain obscure. In this study, a rice mutant with incomplete male sterility was identified. Cytological analyses revealed that this mutant was defective in anther development. Cloning of the mutant gene indicated that the responsible mutation was on OsCYP86A9. OsMYB80 is a core transcription factor in the regulation of rice anther development. The expression of OsCYP86A9 was abolished in the anther of osmyb80 mutant. In vivo and in vitro experiments showed that OsMYB80 binds to the MYB-binding motifs in OsCYP86A9 promoter region and regulates its expression. Furthermore, the oscyp86a9 mutant exhibited an impaired suberin deposition in the root, and was more susceptible to drought stress. Interestingly, genetic and biochemical analyses revealed that OsCYP86A9 expression was regulated in the root by certain MYB transcription factors other than OsMYB80. Moreover, mutations in the MYB genes that regulate OsCYP86A9 expression in the root did not impair the male fertility of the plant. Taken together, these findings revealed the critical roles of OsCYP86A9 in plant development and proposed that OsCYP86A9 functions in anther development and root suberin formation via two distinct tissue-specific regulatory pathways.

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