OsCOL5 suppresses heading through modulation of Ghd7 and Ehd2, enhancing rice yield

文献类型： 外文期刊

第一作者： Wen, Xiaoxia

作者： Wen, Xiaoxia;Zhong, Zhengzheng;Xu, Peng;Yang, Qinqin;Liu, Ling;Wu, Zhaozhong;Wu, Yewen;Zhang, Yingxin;Liu, Qunen;Zhou, Zhengping;Peng, Zequn;Cheng, Shihua;Cao, Liyong;Zhan, Xiaodeng;Wu, Weixun;Wen, Xiaoxia;Zhong, Zhengzheng;Xu, Peng;Yang, Qinqin;Liu, Ling;Wu, Zhaozhong;Wu, Yewen;Zhang, Yingxin;Liu, Qunen;Zhou, Zhengping;Peng, Zequn;Cheng, Shihua;Cao, Liyong;Zhan, Xiaodeng;Wu, Weixun;Wen, Xiaoxia;Liu, Ling;He, Yuqing;Wang, Yinping;Wang, Yinping

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 7 期

页码：

收录情况： SCI

摘要： Heading date, also known as flowering time, plays a crucial role in determining the adaptability and yield potential of rice (Oryza sativa L.). CONSTANS (CO)-like is one of the most critical flowering-associated gene families, members of which are evolutionarily conserved. Here, we report the molecular functional characterization of OsCOL5, an ortholog of Arabidopsis COL5, which is involved in photoperiodic flowering and influences rice yield. Structural analysis revealed that OsCOL5 is a typical member of CO-like family, containing two B-box domains and one CCT domain. Rice plants overexpressing OsCOL5 showed delayed heading and increases in plant height, main spike number, total grain number per plant, and yield per plant under both long-day (LD) and short-day (SD) conditions. Gene expression analysis indicated that OsCOL5 was primarily expressed in the leaves and stems with a diurnal rhythm expression pattern. RT-qPCR analysis of heading date genes showed that OsCOL5 suppressed flowering by up-regulating Ghd7 and down-regulating Ehd2, consequently reducing the expression of Ehd1, Hd3a, RFT1, OsMADS14, and OsMADS15. Yeast two-hybrid experiments showed direct interactions of OsCOL5 with OsELF3-1 and OsELF3-2. Further verification showed specific interactions between the zinc finger/B-box domain of OsCOL5 and the middle region of OsELF3-1 and OsELF3-2. Yeast one-hybrid assays revealed that OsCOL5 may bind to the CCACA motif. The results suggest that OsCOL5 functions as a floral repressor, playing a vital role in rice's photoperiodic flowering regulation. This gene shows potential in breeding programs aimed at improving rice yield by influencing the timing of flowering, which directly impacts crop productivity.

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