Development of an efficient extraction and enrichment method for total flavonoids compounds from Erigeron breviscapus using ultrasound-assisted extraction and macroporous resin adsorption
文献类型： 外文期刊
第一作者： Li, Yang
作者： Li, Yang;Li, Qifeng;Zhang, Jiayu;Xiong, Ranhua;Huang, Chaobo;Zhao, Wei;Yang, Anquan;Xie, Min
作者机构：
关键词： Adsorption; antioxidant; Erigeron breviscapus; extraction; macroporous resin; total flavonoids
期刊名称：PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY （ 影响因子：1.9； 五年影响因子：2.4 ）
ISSN： 1082-6068
年卷期： 2025 年
页码：
收录情况： SCI
摘要： Erigeron breviscapus, a medicinal plant rich in flavonoids with diverse pharmacological properties is an important resource. To maximize the utilization of these valuable bioactive compounds, ultrasound-assisted extraction combined with response surface methodology was used to optimize the extraction conditions for total flavonoids. After comparing six macroporous resins' adsorption and desorption characteristics, AB-8 was selected for enriching total flavonoids, and its adsorption kinetics and thermodynamics were investigated. The following parameters were chosen as optimal for the dynamic adsorption/desorption experiments using an AB-8 filled column: sample loading concentration of 1.14 mg/mL, sample loading flow rate of 2 BV/h, elution solvent of 70% ethanol, desorption flow rate of 1 BV/h, elution solvent volume of 5.5 BV, pH of 2, temperature of 25 degrees C. After enrichment, the concentration of flavonoids was 5.16 times higher than that of crude extract. LC-MS identified 54 flavonoid molecules with excellent antioxidant properties.
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