Proficiency testing for event-specific quantification of genetically modified maize MON87427: Performance assessment based on the metrologically traceable reference values as assigned values

文献类型： 外文期刊

第一作者： Wang, Di

作者： Wang, Di;Fei, Yue;Niu, Chunyan;Lu, Song;Chen, Xian;Gao, Yunhua;Wu, Yuhua;He, Ping;Zhang, Xiujie;Chen, Hong;Wang, Haoqian

作者机构： Natl Inst Metrol, Ctr Adv Measurement Sci, Beijing 100029, Peoples R China;Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Agr Genet Modified Organisms Traceabil Min, Minist Agr & Rural Affairs, Wuhan 430062, Peoples R China;China Natl Accreditat Serv Conform Assessment CNAS, Specialized Stand Accreditat Dept, SSAD, Beijing 100062, Peoples R China;Minist Agr & Rural Affairs MARA, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China

关键词： Proficiency testing; Digital PCR; GM content; Assigned value; Measurement uncertainty

期刊名称：FOOD CHEMISTRY （ 2023影响因子：8.5； 五年影响因子：8.2 ）

ISSN： 0308-8146

年卷期： 2024 年 453 卷

页码：

收录情况： SCI

摘要： The Asia Pacific Metrology Program and the Accreditation Cooperation joint Proficiency Testing (PT) program for the quantification of genetically modified maize MON87427 was organized by the National Institute of Metrology, China, to enhance the measurement accuracy and metrological traceability in the region. Certified reference materials were employed as test samples; metrologically traceable certified reference values served as PT reference values (PTRVs) for evaluating the participants results. The consensus values obtained from the participants were higher than the assigned values, potentially due to the systematic effects of DNA extraction process. The participants' relatively poor overall performance by the zeta-score compared with z-score demonstrates their need to thoroughly investigate quantification bias to elevate the measurement capability of genetically modified (GM) content and deepen their understanding of uncertainty estimation. This program confirmed the importance of using metrologically traceable reference values instead of consensus values as PTRV for reliable performance assessment.

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