Novel Phosphate Transporter-B PvPTB1;1/1;2 Contribute to Efficient Phosphate Uptake and Arsenic Accumulation in As-Hyperaccumulator Pteris vittata

文献类型： 外文期刊

第一作者： Sun, Dan

作者： Sun, Dan;Zhang, Xiang;Zeng, Zihan;Feng, Huayuan;Tang, Yetao;Qiu, Rongliang;Cao, Yue;Feng, Huayuan;Tang, Yetao;Cao, Yue;Yin, Zhibin;Guo, Nan;Tang, Yetao;Qiu, Rongliang;Cao, Yue;Qiu, Rongliang;Qiu, Rongliang;Ma, Lena Q.

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关键词： phytoremediation; low-affinity P transporters PvPTB1;1/1;2; dual P uptakesystem; high-affinity P transporter Pht1; phytatesecretion and hydrolysis; P/As transporters

期刊名称：ENVIRONMENTAL SCIENCE & TECHNOLOGY （ 影响因子：11.4； 五年影响因子：12.0 ）

ISSN： 0013-936X

年卷期： 2024 年 58 卷 17 期

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收录情况： SCI

摘要： Arsenic (As) contamination in soil poses a potential threat to human health via crop uptake. As-hyperaccumulator Pteris vittata serves as a model plant to study As uptake and associated mechanisms. This study focuses on a novel P/AsV transport system mediated by low-affinity phosphate transporter-B 1 family (PTB1) in P. vittata. Here, we identified two plasma-membrane-localized PTB1 genes, PvPTB1;1/1;2, in vascular plants for the first time, which were 4.4-40-fold greater in expression in P. vittata than in other Pteris ferns. Functional complementation of a yeast P-uptake mutant and enhanced P accumulation in transgenic Arabidopsis thaliana confirmed their role in P uptake. Moreover, the expression of PvPTB1;1/1;2 facilitated the transport and accumulation of As in both yeast and A. thaliana shoots, demonstrating a comparable AsV uptake capacity. Microdissection-qPCR analysis and single-cell transcriptome analysis collectively suggest that PvPTB1;1/1;2 are specifically expressed in the epidermal cells of P. vittata roots. PTB1 may play a pivotal role in efficient P recycling during phytate secretion and hydrolysis in P. vittata roots. In summary, the dual P transport mechanisms consisting of high-affinity Pht1 and low-affinity PTB1 may have contributed to the efficient P/As uptake in P. vittata, thereby contributing to efficient phytoremediation for As-contaminated soils.

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