Gap-enhance Raman tags (GERTs) competitive immunoassay based Raman imaging for the quantitative detection of trace florfenicol in milk

文献类型： 外文期刊

第一作者： Shan, Jiajia

作者： Shan, Jiajia;Li, Xinjing;Ren, Tao;Jin, Mengke;Wang, Xue;Han, Shuqing

作者机构：

关键词： Gap-enhance Raman tags (GERTs); Florfenicol; SERS; Immunoassay; Raman mapping

期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）

ISSN： 0308-8146

年卷期： 2022 年 391 卷

页码：

收录情况： SCI

摘要： Surface Enhanced Raman Spectroscopy (SERS) is a highly sensitive detection method, however, it is still challenging to directly detect florfenicol due to its low Raman activity. To solve this problem, we present a novel sensor for selective detection of florfenicol residue in milk using gap-enhance Raman tags (GERTs) competitive immunoassay. In this nanosensor, Raman reporters 4-mercaptobenzoic acid (4MBA) were embedded in the 1 nm gap of Au core-gap-Au shell nanoparticles, which can protect 4MBA molecules from unwanted external environments and provide a high enhancement. The parameters affecting the detection sensitivity were discussed and optimized. The limit detection of florfenicol in milk can reach 0.1 mu g/kg. To evaluate the performance of GERTs immunoassay, Ag@4MBA tags immunoassay was conducted and the results were compared. Moreover, Raman mapping was investigated to improve the quantitative prediction model with R-2 of 0.981 achieved, by eliminating the signal fluctuations caused by the inhomogeneous distribution of GERTs.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序