CRISPR/Cas12a-assisted rapid identification of key beer spoilage bacteria

文献类型： 外文期刊

第一作者： Meng, Qingzhou

作者： Meng, Qingzhou;Leng, Qibin;Yang, Hongmei;Zhang, Guiquan;Liu, Jianghuai;Zhang, Guiquan;Liu, Jianghuai;Sun, Wenjun;Li, Guanglei;Huang, Xingxu;Ma, Peixiang;Liu, Xinyi;Dang, Lu;Wang, Xinjie

作者机构：

关键词： Key beer spoilage bacteria; CRISPR; Cas12a; Nucleic acid detection; Lactobacillus; Pediococcus

期刊名称：INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES （ 影响因子：5.916； 五年影响因子：6.159 ）

ISSN： 1466-8564

年卷期： 2021 年 74 卷

页码：

收录情况： SCI

摘要： Beer spoilage bacteria present severe challenges for the beer fermentation industry. Here, we establish a rapid, specific and highly portable CRISPR/Cas12a-based key beer spoilage bacterial scanning method, dubbed CRISPRBeer Scan. With the variable 16S rDNA segments of these major spoilage microbe species serving as targets, highly efficient and specific crRNAs were determined. By coupling with recombinase polymerase isothermal amplification, CRISPR-Beer Scan could detect as low as 10 copies of DNA targets. Indeed, trace amounts of target genome DNAs can be accurately identified within a mixture of genome DNAs conditioned in beer. Moreover, the extracted genome DNA samples can be conveniently distinguished through the visual fluorescent signals excited by blue light in 45 min with CRISPR-Beer Scan Method. Taken together, the CRISPR-Beer Scan represents a widely applicable microbe-monitoring platform for not only breweries, but also other industries.

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