The decreased expression of GW2 homologous genes contributed to the increased grain width and thousand-grain weight in wheat-Dasypyrum villosum 6VS center dot 6DL translocation lines

文献类型： 外文期刊

第一作者： Feng, Zhiyu

作者： Feng, Zhiyu;Song, Long;Song, Wanjun;Qi, Zhongqi;Yuan, Jun;Li, Run;Chen, Zhaoyan;Guo, Weilong;Xin, Mingming;Liu, Jie;Hu, Zhaorong;Peng, Huiru;Yao, Yingyin;Sun, Qixin;Ni, Zhongfu;Xing, Jiewen;Feng, Zhiyu;Song, Long;Song, Wanjun;Qi, Zhongqi;Yuan, Jun;Li, Run;Chen, Zhaoyan;Guo, Weilong;Xin, Mingming;Liu, Jie;Hu, Zhaorong;Peng, Huiru;Yao, Yingyin;Sun, Qixin;Ni, Zhongfu;Xing, Jiewen;Feng, Zhiyu;Song, Long;Song, Wanjun;Qi, Zhongqi;Yuan, Jun;Li, Run;Chen, Zhaoyan;Guo, Weilong;Xin, Mingming;Liu, Jie;Hu, Zhaorong;Peng, Huiru;Yao, Yingyin;Sun, Qixin;Ni, Zhongfu;Xing, Jiewen;Feng, Zhiyu;Han, Haiming;Wang, Huifang

作者机构：

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2021 年 134 卷 12 期

页码：

收录情况： SCI

摘要： Key message This study demonstrated that the aberrant transcription of DvGW2 contributed to the increased grain width and thousand-grain weight in wheat-Dasypyrum villosum T6VS center dot 6DL translocation lines. Due to the high immunity to powdery mildew, Dasypyrum villosum 6VS has been one of the most successful applications of the wild relatives in modern wheat breeding. Along with the desired traits, side-effects could be brought when large alien chromosome fragments are introduced into wheat, but little is known about effects of 6VS on agronomic traits. Here, we found that T6VS center dot 6DL translocation had significantly positive effects on grain weight, plant heightand spike length, and small negative effects on total spikelet number and spikelet compactness using recipient and wheat-D. villosum T6VS center dot 6DL allohexaploid wheats, Wan7107 and Pm97033. Further analysis showed that the 6VS segment might exert direct genetic effect on grain width, then driving the increase of thousand-grain weight. Furthermore, comparative transcriptome analysis identified 2549 and 1282 differentially expressed genes (DEGs) and 2220 and 1496 specifically expressed genes (SEGs) at 6 days after pollination (DAP) grains and 15 DAP endosperms, respectively. Enrichment analysis indicated that the process of cell proliferation category was over-represented in the DEGs. Notably, two homologous genes, TaGW2-D1 and DvGW2, were identified as putative candidate genes associated with grain weight and yield. The expression analysis showed that DvGW2 had an aberrant expression in Pm97033, resulting in significantly lower total expression level of GW2 than Wan7107, which drives the increase of grain weight and width in Pm97033. Collectively, our data indicated that the compromised expression of DvGW2 is critical for increased grain width and weight in T6VS center dot 6DL translocation lines.

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