Selection of Reference Genes for RT-qPCR Analysis of Wing Dimorphism in English Grain Aphid, Sitobion avenae (Hemiptera: Aphididae)
文献类型： 外文期刊
第一作者： Li, Xiangrui
作者： Li, Xiangrui;Li, Xinan;Chao Wang;Zhang, Yunhui;Xun Zhu;Li, Xinan;Yan, Weiwei;Coates, Brad;Zhou, Xuguo;Gao Haifeng
作者机构：
关键词： Sitobion avenae; wing polyphenism; reference gene; RT-qPCR; normalization
期刊名称：JOURNAL OF ECONOMIC ENTOMOLOGY （ 影响因子：2.447； 五年影响因子：2.483 ）
ISSN： 0022-0493
年卷期： 2022 年 115 卷 1 期
页码：
收录情况： SCI
摘要： The English grain aphid, Sitobion avenae (Fabricius), exhibits classic and dramatic phenotypic plasticity in wing development. Both genetic and environmental inputs contribute to the wing polyphenism in aphids, an extreme form of phenotypic plasticity in which a single genotype produces discrete winged and wingless morphs. Validated reference genes are needed to accurately normalize temporal and spatial variation in gene expression estimates by RT-qPCR. In this research, the stability of 11 candidate reference genes selected from S. avenae transcriptomes was evaluated under an array of abiotic and biotic conditions relevant to wing development. RefFinder, a comprehensive software integrating rankings from delta Ct, BestKeeper, NormFinder, and geNorm, offered a series of reference genes for every experimental condition. Overall, helicase (HEL) and ubiquitin ribosomal protein S27A fusion protein (RpS27) are suited for most of the conditions examined in this study, although exceptions do exist. Specifically, NADH dehydrogenase (Ap-NADH) and 28S ribosomal RNA (28S) are recommended for insecticide and antibiotic treatments, while ribosomal RNA L14 (RPL14) and 18S ribosomal RNA (18S) are selected for density treatment, respectively. This study provides a suite of reference genes to investigate the wing polyphenism in S. avenae, and is important for application of RT-qPCR in future experiments of novel tactics to control aphids.
分类号：
- 相关文献
作者其他论文 更多>>
-
The development of an egg-soaking method for delivering dsRNAs into spider mites
作者：Yang, Jing;Zhang, Yuying;Zhang, Zhonghuan;Ren, Meifeng;Wang, Yifei;Duan, Yuanpeng;Gao, Yue;Liu, Zhongfang;Zhang, Pengjiu;Fan, Renjun;Zhou, Xuguo
关键词：dsRNA delivery; Egg-soaking RNAi method; Sprayable RNAi biopesticide; Spider mites; Amphitetranychus viennensis; Tetranychus urticae
-
Uridine diphosphate glucosyltransferases are involved in spinosad resistance in western flower thrips Frankliniella occidentalis (Pergande)
作者：Wang, Jing;Wan, Yanran;Zhang, Ying;Yuan, Jiangjiang;Zheng, Xiaobin;Cao, Hongyi;Qian, Kanghua;Feng, Jiuming;Tang, Yingxi;Chen, Sirui;Zhang, Youjun;Wu, Qingjun;Wang, Jing;Liang, Pei;Zhou, Xuguo
关键词：UGT detoxification system; Pesticide resistance; Upregulated gene expression; Gene duplication; Dose-dependent
-
Single-cell transcriptome landscape elucidates the cellular and developmental responses to tomato chlorosis virus infection in tomato leaf
作者：Yue, Hao;Zhang, Zhuo;Zhang, Songbai;Peng, Jing;Gao, Yang;Zhang, Deyong;Shi, Xiaobin;Liu, Yong;Yue, Hao;Zhang, Deyong;Shi, Xiaobin;Liu, Yong;Chen, Gong;Guo, Zhaojiang;Zhang, Zhanhong;Turlings, Ted C. J.;Zhou, Xuguo
关键词：cell atlas; single-cell RNA sequencing (scRNA-seq); Solanum lycopersicum; ToCV infection; transcriptional factor
-
Effects of miR-306 Perturbation on Life Parameters in the English Grain Aphid, Sitobion avenae (Homoptera: Aphididae)
作者：Wu, Linyuan;Wei, Guohua;Yan, Yi;Zhu, Xun;Zhang, Yunhui;Li, Xiangrui;Zhou, Xuguo
关键词：Sitobion avenae; miR-306; growth and development; two-sex life table; population prediction
-
A Novel Allele in the Promoter of Wx Decreases Gene Expression and Confers Lower Apparent Amylose Contents in Japonica Rice (Oryza sativa L.)
作者：Tang, Weijie;Chen, Haiyuan;Zhang, Suobing;Tang, Jun;Lin, Jing;Fang, Xianwen;Zhang, Yunhui;Tang, Weijie;Chen, Haiyuan;Zhang, Suobing;Tang, Jun;Lin, Jing;Fang, Xianwen;Zhang, Yunhui;Chen, Gaoming;Zhang, Yunhui
关键词：Wx; promoter; allele; AAC; japonica rice; nitrogen
-
Effects of 1-methylcyclopropene on skin greasiness and quality of 'Yuluxiang' pear during storage at 20°C
作者：Yu, Wanting;Zhang, Xinnan;Yan, Weiwei;Sun, Xiaonan;Wang, Yang;Jia, Xiaohui
关键词：'Yuluxiang' pear; skin greasiness; 1-methylcyclopropene; storage quality
-
RNA m6A Methylation Suppresses Insect Juvenile Hormone Degradation to Minimize Fitness Costs in Response to A Pathogenic Attack
作者：Guo, Zhaojiang;Bai, Yang;Zhang, Xinyi;Guo, Le;Zhu, Liuhong;Sun, Dan;Sun, Kaiyue;Xu, Xudan;Yang, Xin;Xie, Wen;Wang, Shaoli;Wu, Qingjun;Zhang, Youjun;Crickmore, Neil;Zhou, Xuguo
关键词：Bacillus thuringiensis; host-pathogen interactions; juvenile hormone esterase; m(6)A modification; Plutella xylostella
