Temperature-responsive deep eutectic solvents as eco-friendly and recyclable media for microwave extraction of flavonoid compounds from waste onion (Allium cepa L.) skins
文献类型： 外文期刊
第一作者： Shang, Xian-chao
作者： Shang, Xian-chao;Zheng, Yan-fen;Li, Yiqiang;Shang, Xian-chao;Zhang, Yu-qin
作者机构：
关键词： Temperature-responsive deep eutectic solvents; Spent onion skins; Flavonoids; Box-Behnken design; Antioxidant activity
期刊名称：BIOMASS CONVERSION AND BIOREFINERY （ 影响因子：4.05； 五年影响因子：4.103 ）
ISSN： 2190-6815
年卷期：
页码：
收录情况： SCI
摘要： Polyphenols and flavonoids are abundant in spent onion skins (SOSs), being present in amounts are 2-sixfold greater times of than those in the inner bulb. In this study, an eco-friendly and efficient extraction method based on temperature-responsive deep eutectic solvents (TRDESs) was used to obtain natural flavonoids from SOSs. Microwave-assisted extraction yields of quercetin, kaempferol, luteolin, and quercetin-3-O-beta-D-glucoside were evaluated to determine the efficiencies of a series of TRDESs. An extraction system composed of ethanolamine and m-cresol at ratio of 1:1 (20% water in TRDES system) was found to possess the highest efficiency. The extraction conditions were screened and optimized for efficiency using a Box-Behnken design. As a result, an extraction power of 554 W, extraction temperature of 76 degrees C, extraction time of 16 min, and solid-liquid ratio of 14 mg/mL were determined to be the optimal conditions. Under these extraction conditions, the total flavonoid extraction yields reached 47.83 +/- 0.21 mg/g. The macroporous resin HPD 600 was selected for the separation and recovery of the TRDESs extraction mixture and the experimental measurements revealed that it allowed recovery rate more than 90%. In vitro activity testing of the flavonoid extract demonstrated excellent FRAP assay, DPPH assay and ABTS assay results for the SOSs extract prepared using a TRDES, and its inhibitory effects on S. aureus, E. carotovora, and B. subtilis were marked.
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