Generation of APN-chimeric gene-edited pigs by CRISPR/Cas9-mediated knock-in strategy

文献类型： 外文期刊

第一作者： Liu, Zhenying

作者： Liu, Zhenying;Zhang, Mingjun;Huang, Peixuan;Qi, Chunyun;Jiao, Shuyu;Zhao, Dehua;Jiang, Yuan;Chen, Xue;Lv, Dongmei;Pang, Daxin;Xie, Zicong;Ouyang, Hongsheng;Pang, Daxin;Ouyang, Hongsheng;Pang, Daxin;Ouyang, Hongsheng;Ouyang, Hongsheng;Ji, Zhaoyang;Zhang, Xin;Feng, Li;Xie, Zicong;Ouyang, Hongsheng

作者机构：

关键词： TGEV; APN; CRISPR; Cas9; Knock-in; Gene-edited pigs

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 851 卷

页码：

收录情况： SCI

摘要： The prevalence of porcine enteric coronaviruses (PECs), including transmissible gastroenteritis virus (TGEV), swine acute diarrhea syndrome coronavirus (SADS-CoV), porcine delta coronavirus (PDCoV), and porcine epidemic diarrhea virus (PEDV), poses a serious threat to animal and public health. Here, we aimed to further optimize the porcine aminopeptidase N (pAPN) gene editing strategy to explore the balance between individual antiviral properties and the biological functions of pAPN in pigs. Finally, APN-chimeric gene-edited pigs were produced through a CRISPR/Cas9-mediated knock-in strategy. Further reproductive tests indicated that these gene-edited pigs exhibited normal pregnancy rates and viability. Notably, in vitro viral challenge assays further demonstrated that porcine kidney epithelial cells isolated from F1-generation gene-edited pigs could effectively inhibit TGEV infection. This study is the first to report the generation of APN-chimeric pigs, which may provide a natural host animal for characterizing PEC infection with APN and help in the development of better antiviral solutions.

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