Comparative transcriptome profiling of Eimeria tenella in various developmental stages and functional analysis of an ApiAP2 transcription factor exclusively expressed during sporogony

文献类型： 外文期刊

第一作者： Chen, Linlin

作者： Chen, Linlin;Sun, Pei;Chen, Junmin;Liu, Jie;Gao, Yang;Hao, Zhenkai;Zhang, Ning;Chen, Wenxuan;Xie, Fujie;Suo, Xun;Liu, Xianyong;Chen, Linlin;Sun, Pei;Chen, Junmin;Liu, Jie;Gao, Yang;Hao, Zhenkai;Zhang, Ning;Chen, Wenxuan;Xie, Fujie;Suo, Xun;Liu, Xianyong;Tang, Xinming;Hu, Dandan;Zhang, Yuanyuan;Zhang, Yuanyuan;Wang, Chaoyue

作者机构：

关键词： Eimeria tenella; Life cycle; Transcriptome; ApiAP2 transcription factor; Gene editing

期刊名称：PARASITES & VECTORS （ 影响因子：3.2； 五年影响因子：3.6 ）

ISSN： 1756-3305

年卷期： 2023 年 16 卷 1 期

页码：

收录情况： SCI

摘要： Background The apicomplexan parasites Eimeria spp. are the causative agents of coccidiosis, a disease with a significant global impact on the poultry industry. The complex life cycle of Eimeria spp. involves exogenous (sporogony) and endogenous (schizogony and gametogony) stages. Unfortunately, the genetic regulation of these highly dynamic processes, particularly for genes involved in specific developmental phases, is not well understood.Methods In this study, we used RNA sequencing (RNA-Seq) analysis to identify expressed genes and differentially expressed genes (DEGs) at seven time points representing different developmental stages of Eimeria tenella. We then performed K-means clustering along with co-expression analysis to identify functionally enriched gene clusters. Additionally, we predicted apicomplexan AP2 transcription factors in E. tenella using bioinformatics methods. Finally, we generated overexpression and knockout strains of ETH2_0411800 to observe its impact on E. tenella development.ResultsIn total, we identified 7329 genes that are expressed during various developmental stages, with 3342 genes exhibiting differential expression during development. Using K-means clustering along with co-expression analysis, we identified clusters functionally enriched for oocyte meiosis, cell cycle, and signaling pathway. Among the 53 predicted ApiAP2 transcription factors, ETH2_0411800 was found to be exclusively expressed during sporogony. The ETH2_0411800 overexpression and knockout strains did not exhibit significant differences in oocyst size or output compared to the parental strain, while the resulting ETH2_0411800 knockout parasite showed a relatively small oocyst output.Conclusions The findings of our research suggest that ETH2_0411800 is not essential for the growth and development of E. tenella. Our study provides insights into the gene expression dynamics and is a valuable resource for exploring the roles of transcription factor genes in regulating the development of Eimeria parasites.

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