Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22

文献类型： 外文期刊

第一作者： Xu, W. G.

作者： Xu, W. G.;Li, C. X.;Hu, L.;Zhang, L.;Zhang, J. Z.;Dong, H. B.;Wang, G. S.;Li, C. X.

作者机构：

关键词： Infection;Molecular Genetics: Biochemistry and Molecular Biophysics;Agronomy: Agriculture

期刊名称：MOLECULAR BREEDING （ 影响因子：2.589； 五年影响因子：2.75 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The Chinese winter wheat cultivar Zhoumai 22 is highly resistant to powdery mildew. The objectives of this study were to map a powdery mildew resistance gene in Zhoumai 22 using molecular markers and investigate its allelism with Pm13. A total of 278 F-2 and 30 BC1 plants, and 143 F-3 lines derived from the cross between resistant cultivar Zhoumai 22 and susceptible cultivar Chinese Spring were used for resistance gene tagging. The 137 F-2 plants from the cross Zhoumai 22/2761-5 (Pm13) were employed for the allelic test of the resistance genes. Two hundred and ten simple sequence repeat (SSR) markers were used to test the two parents, and resistant and susceptible bulks. Subsequently, seven polymorphic markers were used for genotyping the F-2 and F-3 populations. The results indicated that the powdery mildew resistance in Zhoumai 22 was conferred by a single dominant gene, designated PmHNK tentatively, flanked by seven SSR markers Xgwm299, Xgwm108, Xbarc77, Xbarc84, Xwmc326, Xwmc291 and Xwmc687 on chromosome 3BL. The resistance gene was closely linked to Xwmc291 and Xgwm108, with genetic distances of 3.8 and 10.3 cM, respectively, and located on the chromosome bin 3BL-7-0.63-1.0 in the test with a set of deletion lines. Seedling tests with seven isolates of Blumeria graminis f. sp. tritici (Bgt) and allellic test indicated that PmHNK is different from Pm13, and Pm41 seems also to be different from PmHNK due to its origin from T. dicoccoides and molecular evidence. These results indicate that PmHNK is likely to be a novel powdery mildew resistance gene in wheat.

分类号： Q94

  • 相关文献

[1]Molecular Cloning and Expression of cDNA Encoding the Cysteine Proteinase Inhibitor from Upland Cotton. Jiang, Ming-feng,Li, Sheng-wei,Chen, Min,Li, Sheng-wei,Chen, Min,Cai, Ying-fan,Xie, Yong-fang,Li, Biao,Sun, Quan,Jiang, Huai-zhong,Pan, Zheng,Gao, Yun-ling,Yuan, You-Lu,Shi, Yu-zheng.

[2]Characterization of Five Putative Acyl Carrier Protein (ACP) Isoforms from Developing Seeds of Arachis hypogaea L.. Li, Meng-Jun,Wang, Xing-Jun,Su, Lei,Bi, Yu-Ping,Wan, Shu-Bo.

[3]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

[4]OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: implication for a highly conserved plant cGTPase essential for chloroplast function. Liu, Hongjia,Li, Sujuan,Huang, Guo,Guo, Peng,Tao, Yuezhi,Liu, Hongjia,Chu, Hung,Lo, Clive,Lau, Edward,Lam, Maggie P. Y.,Chu, Ivan K.,Wang, Junqi,Jiang, Liwen.

[5]The association of Sclerotinia sclerotiorum resistance with glucosinolates in Brassica napus double-low DH population. Fan, Z. X.,Sun, X. L.,Yu, B.,Wang, Y. Z.,Yang, G. S.,Lei, W. X.,Fan, Z. X..

[6]Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus Derived from Wild Pikas in China. Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Wu, Nanping,Li, Lanjuan,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yan, Yan,Chen, Hualan,Liao, Ming,Luo, Kaijian,Huang, Yu.

[7]'Candidatus Phytoplasma tamaricis', a novel taxon discovered in witches'-broom-diseased salt cedar (Tamarix chinensis Lour.). Zhao, Yan,Sun, Qingrong,Wei, Wei,Davis, Robert E.,Wu, Wei,Liu, Qingzhong,Sun, Qingrong,Liu, Qingzhong.

[8]Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Sun, Yuan,Liu, Da-Fei,Wang, Yu-Fei,Liang, Bing-Bing,Cheng, Dan,Li, Na,Qi, Qiao-Fen,Zhu, Qing-Hu,Qiu, Hua-Ji.

[9]An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8(+) cytotoxic T cell response. Cai, Hong,Hu, Xi-Dan.

[10]A TaqMan real-time PCR assay for quantifying white spot syndrome virus (WSSV) infections in wild broodstock and hatchery-reared postlarvae of fleshy shrimp, Fenneropenaeus chinensis. Meng, Xian-Hong,Jang, In-Kwon,Meng, Xian-Hong,Seo, Hyung-Chul,Cho, Yeong-Rok,Kim, Bong-Rae,Ayyaru, Gopalakannan,Kim, Jong-Sheek.

[11]Analysis of Quantitative Trait Loci for Panicle Layer Uniformity in Rice (Oryza sativa L.). Ma, L. Y.,Xiao, P.,Xia, Y. W.,Bao, J. S.,Ma, L. Y.,Xiao, P.,Xia, Y. W.,Bao, J. S.,Ma, L. Y.,Cai, J.,Li, X. M.,Ji, Z. J.,Yang, C. D..

[12]Transgenic Wheat Plants Derived from Agrobacterium-mediated Transformation of Mature Embryo Tissues. Wang, Y. L.,Yin, G. X.,Tao, L. L.,Ye, X. G.,Xu, M. X.,Wang, D. W..

[13]Field-Testing of Synthetic Herbivore-Induced Plant Volatiles as Attractants for Beneficial Insects. Zhang, Yongjun,Wu, Kongming,Guo, Yu Yuan,Yu, Huilin.

[14]Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts. Li, Shangang,Yin, Chunguang,Xu, Lingyang,Liu, Tao,Li, Hongbin,Du, Lixin,Guo, Yi,Shi, Jianjun,Xing, Fengying,Li, Yao,Chen, Xuejin,Zhang, Chuanshan.

[15]Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. Dang, Zhisheng,Kajino, Kiichi,Nakao, Ryo,Sugimoto, Chihiro,Dang, Zhisheng,Yagi, Kinpei,Kouguchi, Hirokazu,Oku, Yuzaburo,Matsumoto, Jun,Watanabe, Junichi,Matsumoto, Jun,Wakaguri, Hiroyuki,Toyoda, Atsushi,Toyoda, Atsushi.

[16]A simple artificial microRNA vector based on ath-miR169d precursor from Arabidopsis. Liu, Chong,Zhang, Lan,Luo, Yanzhong,Fan, Yun-Liu,Wang, Lei,Liu, Chong,Sun, Jie,Wang, Ming-Bo.

[17]Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells. Yang, Run Jun,Li, Jun Ya,Zhang, Lu Pei,Gao, Xue,Chen, Jin Bao,Xu, Shang Zhong.

[18]Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin. Lu, Hong-Yu,Zhang, Hai-Chao,Gao, Shan-Lin,Liu, Jing-Mei,Yin, Tao.

[19]The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Guan, Guiquan,Luo, Jianxun,Liu, Zhijie,Gao, Jinliang,Ma, Miling,Liu, Aihong,Dang, Zhisheng,Liu, Junlong,Ren, Qiaoyun,Jin, Yurong,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Inoue, Noboru,Thekisoe, Oriel M. M.,Sugimoto, Chihiro.

[20]RGA-ILP, a new type of functional molecular markers in bread wheat. Shang, World,Zhou, Ronghua,Jia, Jizeng,Gao, Lifeng.

作者其他论文 更多>>

微信小程序