Molecular analysis of grass carp (Ctenopharyngodon idella) by SRAP and SCAR molecular markers

文献类型： 外文期刊

第一作者： Ding, Wei-dong

作者： Ding, Wei-dong;Cao, Zhe-ming;Cao, Li-ping

作者机构：

关键词： artificial selection;genetic frequency;germplasm identification trait;Genome Sequence Survey database

期刊名称：AQUACULTURE INTERNATIONAL （ 影响因子：2.235； 五年影响因子：2.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The sequence-related amplified polymorphism (SRAP) technique was used to analyze the gene differentiation between two cultured populations [Freshwater Fisheries Research Center (FFRC) and Qianzhou populations] and one wild population (Hanjian population) of grass carp (Ctenopharyngodon idella). Some loci showed quite different genetic frequencies, attributable to artificial selection, which imply that these fragments are putative markers of germplasm identification. We developed a simple and effective method to further characterize these SRAP fragments. Specific SRAP bands were cut directly from polyacrylamide gels, re-amplified, cloned, and sequenced. Twenty-one putative genetic markers were sequenced, ranging from 137 to 357 bp. The sequences were submitted to the database of the Genome Sequence Survey. A BLAST analysis showed that eight SRAP fragments were highly similar to functional genes, whereas the other 13 had no similarity, indicating that these markers are tightly linked to the germ identification trait although only eight are functional genes. Three primers were designed according to this sequence information and used for PCR amplification of the three populations. A sequence-characterized amplified region (SCAR1) was positively amplified in the artificially cultured populations but not in the wild population. The frequency of the SCAR3 marker in the cultured populations was 87% (26/174), whereas it was only 6% (6/100) in the wild population. A specific band was isolated from all individuals in the wild population with the SCAR3 primers, whereas the specific band was amplified from only seven individuals in the FFRC population and from none of the Qianzhou population. The frequency of SCAR2 in the artificially cultured populations was 96.5%. These results indicate that SCAR1 could be used as a specific molecular marker for population identification. The SCAR markers used in this study offer a powerful, easy, and rapid method for genetic analysis and the discrimination of different populations.

分类号： S9

  • 相关文献

[1]The phylogeographic system survey of native sheep breeds in the eastern and southern Central Asia. Sun, W.,Ma, Y. H.,Guan, W. J.,Sun, W.,Chang, H.,Musa, H. H.,Yang, Z. P.,Tsunoda, K.,Musa, H. H..

[2]QTL mapping for fiber quality traits across multiple generations and environments in upland cotton. Fu-Ding Sun,Jian-Hong Zhang,Shu-Fang Wang,Wan-Kui Gong,Yu-Zhen Shi,Ai-Ying Liu,Jun-Wen Li,Ju-Wu Gong,Hai-Hong Shang,You-Lu Yuan.

[3]Analysis of Fitness Predominance for Gaoyou Duck's Double Yolk Egg. Zhang, Tangjie,Chang, Hong,Li, Hui-Fang,Chen, Kuanwei,Zhao, Yonggao,Xuec, Minkai,Zhang, Shengfu. 2011

[4]A genomic perspective on the important genetic mechanisms of upland adaptation of rice. Lyu, Jun,Gou, Zhiheng,Wang, Wen,Li, Baoye,Zhang, Shilai,Zhang, Jing,Tao, Dayun,Huang, Wangqi,Hu, Fengyi,He, Weiming,Meng, Liyun,Li, Xin. 2014

[5]Functional markers in wheat: current status and future prospects. Liu, Yanan,He, Zhonghu,Xia, Xianchun,He, Zhonghu,Appels, Rudi.

[6]A preliminary study for identification of candidate AFLP markers under artificial selection for shell color in pearl oyster Pinctada fucata. Zou, Keshu,Zhang, Dianchang,Guo, Huayang,Zhu, Caiyan,Li, Min,Jiang, Shigui,Zou, Keshu,Zhang, Dianchang,Li, Min,Jiang, Shigui.

[7]Genome-wide association mapping of QTL underlying seed oil and protein contents of a diverse panel of soybean accessions. Li, Ying-hui,Hong, Hui-long,Li, Hui-hui,Liu, Zhang-xiong,Tian, Yun,Li, Yan-fei,Qiu, Li-juan,Reif, Jochen C.,Ma, Yan-song,Li, Jun,Li, Wen-bin. 2018

[8]Evidence of balancing selection in multiple indigenous chicken populations. Arlud, S.,Zeng, S. C.,Arlud, S.,Arlud, S.,E, G. X.. 2016

[9]A non-synonymous SNP within the isopentenyl transferase 2 locus is associated with kernel weight in Chinese maize inbreds (Zea mays L.). Weng, Jianfeng,Liu, Changlin,Hao, Zhuanfang,Li, Mingshun,Zhang, Degui,Ci, Xiaoke,Li, Xinhai,Zhang, Shihuang,Li, Bo,Wang, Hongwei,Yang, Xiaoyan. 2013

[10]OsLG3 contributing to rice grain length and yield was mined by Ho-LAMap. Xiong, Haiyan,Zhu, Xiaoyang,Zhang, Hongliang,Miao, Jinli,Zhang, Zhanying,Yao, Guoxin,Zhang, Qiang,Pan, Yinghua,Wang, Xin,Rashid, M. A. R.,Li, Jinjie,Li, Zichao,Li, Huihui,Wang, Wensheng,Gao, Yongming,Li, Zhikang,Tang, Zuoshun,Yang, Weicai,Fu, Xiangdong,Pan, Yinghua. 2017

[11]Molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing. Li, Ying-hui,Yan, Long,Qi, Xiao-tian,Zhang, Le,Chang, Ru-zhen,Guo, Yong,Wang, Xiao-bo,Guan, Rong-xia,Liu, Yu-lin,Jin, Long-guo,Liu, Zhang-xiong,Zhang, Li-juan,Wang, Ke-jing,Qiu, Li-juan,Zhao, Shan-cen,Li, Dong,Li, Jun,Guo, Xiao-sen,He, Wei-ming,Liang, Qin-si,Ye, Chen,Tao, Yong,Wang, Jun-yi,Zhang, Xiu-qing,Chen, Jie,Nielsen, Rasmus,Li, Rui-qiang,Wang, Jian,Wang, Jun,Ma, Jian-xin,Yan, Long,Zhang, Meng-chen,Tao, Yong,Nielsen, Rasmus,Wang, Jun,Wang, Jun-yi,Nielsen, Rasmus,Nielsen, Rasmus,Chen, Peng-yin,Li, Wen-bin,Reif, Jochen C.,Purugganan, Michael,Purugganan, Michael. 2013

[12]Gene duplication confers enhanced expression of 27-kDa gamma-zein for endosperm modification in quality protein maize. Liu, Hongjun,Sun, Chuanlong,Zheng, Xixi,Yuan, Ningning,Li, Changsheng,Zhang, Zhiyong,Deng, Yiting,Wang, Jiechen,Wu, Yongrui,Shi, Junpeng,Lai, Jinsheng,Shi, Junpeng,Lai, Jinsheng,Gong, Hao,Huang, Xuehui,Feng, Qi,Han, Bin,Fan, Xingming,Qiu, Fazhan,Pan, Guangtang,Pan, Guangtang.

[13]Pedigree-based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding. Zhou, Degui,Wang, Chongrong,Li, Hong,Li, Kanghuo,Zhou, Shaochuan,Chen, Wei,Lin, Zechuan,Chen, Haodong,Yu, Renbo,Zhen, Gang,He, Hang,Deng, Xing Wang,Chen, Wei,Lin, Zechuan,Chen, Haodong,Yu, Renbo,Zhen, Gang,He, Hang,Deng, Xing Wang,Chen, Wei,Chen, Haodong,Yu, Renbo,Zhen, Gang,Tang, Xiaoyan,He, Hang,Deng, Xing Wang,Zhang, Fengyun,Yi, Junliang,Zhou, Shaochuan,Liu, Yaoguang,Terzaghi, William,He, Hang,Deng, Xing Wang.

作者其他论文 更多>>

微信小程序