Construction of recombinant Lactococcus expressing thymosin and interferon fusion protein and its application as an immune adjuvant

文献类型： 外文期刊

第一作者： Liu, Zengqi

作者： Liu, Zengqi;Zhang, Suhua;Hu, Hongjiao;Wang, He;He, Gaoming;Qiu, Yu;Dong, Mingqi;Wang, Muping;Wang, Yunfeng;Cui, Ziyang;Cui, Hongyu

作者机构：

关键词： Thymosin; Interferon; Recombinant Lactococcus; Immune enhancer; Immune adjuvant

期刊名称：MICROBIAL CELL FACTORIES （ 影响因子：6.4； 五年影响因子：6.3 ）

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年卷期： 2024 年 23 卷 1 期

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收录情况： SCI

摘要： Background In recent years, biosafety and green food safety standards have increased the demand for immune enhancers and adjuvants. In the present study, recombinant food-grade Lactococcus lactis (r-L. lactis-T alpha 1-IFN) expressing thymosin T alpha 1 and chicken interferon fusion protein was constructed. Results The in vitro interactions with macrophages revealed a mixture of recombinant r-L. lactis-T alpha 1-IFN could significantly activate both macrophage J774-Dual (TM) NF-kappa B and interferon regulator (IRF) signaling pathways. In vitro interactions with chicken peripheral blood mononuclear cells (PBMCs) demonstrated that a mixture of recombinant r-L. lactis-T alpha 1-IFN significantly enhanced the expression levels of interferon (IFN)-gamma, interleukin (IL)-10, CD80, and CD86 proteins in chicken PBMCs. Animal experiments displayed that injecting a lysis mixture of recombinant r-L. lactis-T alpha 1-IFN could significantly activate the proliferation of T cells and antigen-presenting cells in chicken PBMCs. Moreover, 16S analysis of intestinal microbiota demonstrated that injection of the lysis mixture of recombinant r-L. lactis-T alpha 1-IFN could significantly improve the structure and composition of chicken intestinal microbiota, with a significant increase in probiotic genera, such as Lactobacillus spp. Results of animal experiments using the lysis mixture of recombinant r-L. lactis-T alpha 1-IFN as an immune adjuvant for inactivated chicken Newcastle disease vaccine showed that the serum antibody titers of the experimental group were significantly higher than those of the vaccine control group, and the expression levels of cytokines IFN-gamma and IL-2 were significantly higher than those of the vaccine control group. Conclusion These results indicate that food-safe recombinant r-L. lactis-T alpha 1-IFN has potential as a vaccine immune booster and immune adjuvant. This study lays the foundation for the development of natural green novel animal immune booster or immune adjuvant.

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