Development of Multiple-Heading-Date mtl Haploid Inducer Lines in Rice

文献类型： 外文期刊

第一作者： Wang, Jian

作者： Wang, Jian;Cao, Yuexuan;Wang, Kejian;Liu, Chaolei

作者机构：

关键词： haploid inducers; MTL; multiple heading dates; rice; breeding; DH technology

期刊名称：AGRICULTURE-BASEL （ 影响因子：3.408； 五年影响因子：3.459 ）

ISSN：

年卷期： 2022 年 12 卷 6 期

页码：

收录情况： SCI

摘要： In vivo doubled haploid (DH) production based on crossing heterozygous germplasm with mtl haploid inducer lines promises to transform modern rice (Oryza sativa) breeding. However, this technology is limited, as haploid inducers and pollen acceptors have asynchronous heading dates. To address this obstacle, we developed a panel of multiple-heading-date mtl haploid inducer lines that produce pollen for more than 35 days. We edited the MTL gene in a hybrid rice with the CRISPR-Cas9 system. We then selected transgene-free homozygous mutants in the T-1 generation and reproduced to T-4 generation by single-seed descent method. We obtained 547 mtl haploid inducers with diverse heading dates (from 73 to 110 days) and selected 16 lines comprising a core population with continuous flowering. The seed-setting rate and haploid induction rate (HIR) of the core panel were 4.0-12.7% and 2.8-12.0%, respectively. Thus, our strategy of using multiple-heading-date mtl haploid inducers could accelerate the use of in vivo DH technology in rice breeding.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序