Characteristics and Multilineage Differentiation of a Novel Type of Pulmonary Mesenchymal Stem Cells Derived from Goose Embryos

文献类型： 外文期刊

第一作者： Ma, Caiyun

作者： Ma, Caiyun;Liu, Changqing;Wen, Hebao;Ma, Caiyun;Liu, Changqing;Wen, Hebao;Song, Hanan;Sui, Baoku;Zhang, Yang;Wu, Yue;Wang, Yunan;Guan, Weijun

作者机构：

关键词： PMSCs; Proliferation; Cell differentiation; Biological characterization; Regenerative therapy

期刊名称：BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY （ 影响因子：1.0； 五年影响因子：1.1 ）

ISSN： 1516-8913

年卷期： 2024 年 67 卷

页码：

收录情况： SCI

摘要： Pulmonary mesenchymal stem cells (PMSCs) are considered important in therapeutic and regenerative responses to lung injury. Despite extensive studies on the human, porcine, sheep, mouse and rat, it was found that PMSCs, which can been performed on goose, have not been well explored. Elucidation of cell differentiation, proliferation, which have not yet been well described, may promote the development of injured lung therapy. In our study, we firstly disclosed biological characteristics of spindle-shaped PMSCs from pulmonary tissues of goose embryos. Growth kinetics and counting kit-8 (CCK8) assay were employed for proliferative activity evaluation. When appropriately induced, PMSCs could differentiate into osteoblasts, adipocytes, chondrocytes, type II alveolar epithelial cells (ATII), which demonstrated that PMSCs have crossembryonic layer differentiation potential. Besides, undifferentiated PMSCs consistently expressed MSCs associated phenotypes, such as CD166, CD90, CD44, CD29, CD71 and CD105, as identified by immunohistochemistry and RT-PCR. Karyotype analysis demonstrated that PMSCs possessed stable diploid karyotype. Collectively, we successfully established applicable methods for isolation, culture, identification and characterization of goose PMSCs which suggested a potential therapeutic use in regenerative therapy.

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