RNA-Seq Reveals miRNA and mRNA Co-regulate Muscle Differentiation in Fetal Leizhou Goats

文献类型： 外文期刊

第一作者： Ye, Junning

作者： Ye, Junning;Zhao, Xiuhui;Xue, Huiwen;Liu, Guangbin;Deng, Ming;Sun, Baoli;Guo, Yongqing;Liu, Dewu;Li, Yaokun;Ye, Junning;Zhao, Xiuhui;Zou, Xian;Ye, Junning;Zhao, Xiuhui;Liu, Guangbin;Deng, Ming;Sun, Baoli;Guo, Yongqing;Liu, Dewu

作者机构：

关键词： Leizhou goat; muscle differentiation; miRNA; mRNA; enrichment

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.471； 五年影响因子：3.821 ）

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年卷期： 2022 年 9 卷

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收录情况： SCI

摘要： Muscle differentiation is an essential link in animal growth and development, and microRNA and mRNA are indispensable in skeletal muscle differentiation. To improve the meat quality and production of the Leizhou goat, it is vital to understand the molecular mechanism by which its skeletal muscle differentiates. By RNA sequencing (RNA-SEQ), we established miRNA-mRNA profiles of Leizhou goats at three stages: fetal day 70, 90, and 120. There were 991 differently expressed mRNAs and 39 differentially expressed miRNAs found, with the differentially expressed mRNAs mainly enriched in calcium ion binding, ECM-receptor interaction, and Focal adhesion. CKM and MYH3, two muscle differentiation markers, were significantly differentially expressed during this period. In addition, we found that chi-miR-129-5p, chi-miR-433, and chi-miR-24-3p co-regulate muscle differentiation with their target genes. Finally, we can confirm that muscle differentiation occurred in Leizhou goat between 90 and 120 days of the fetus. This study is helpful to better explore the molecular mechanism of goat muscle differentiation.

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