Discovering Functional Diversity of Lytic Polysaccharide Monooxygenases from the Thermophilic Fungus Myceliophthora Thermophila and Their Application in Lignocellulosic Biomass Degradation

文献类型： 外文期刊

第一作者： Qin, Xing

作者： Qin, Xing;Zou, Jiahuan;Yang, Kun;Li, Jinyang;Wang, Xiaolu;Tu, Tao;Zhang, Jie;Su, Xiaoyun;Yao, Bin;Huang, Huoqing;Luo, Huiying

作者机构：

关键词： lytic polysaccharide monooxygenase; Myceliophthora thermophila; lignocellulosic biomass; glycoside hydrolase; synergistic action

期刊名称：ACS SUSTAINABLE CHEMISTRY & ENGINEERING （ 影响因子：8.4； 五年影响因子：8.7 ）

ISSN： 2168-0485

年卷期： 2023 年 11 卷 29 期

页码：

收录情况： SCI

摘要： Although lytic polysaccharide monooxygenase (LPMO) genesare highlyabundant in the genomes of ascomycetes and basidiomycetes, their biologicalroles in single species for lignocellulose degradation remain unclear.Herein, transcriptomic analysis of the efficient biomass degrader Myceliophthora thermophila grown on corn straw revealeda high number and transcript abundance of LPMOs and glycoside hydrolases(GHs) in response to lignocellulose degradation. To gain a deeperunderstanding of the roles of LPMOs, phenotypic characterization ofsingle gene deletion mutants and catalytic properties of recombinantLPMOs were valuated. Single gene deletion mutants of these highlyexpressed AA9 LPMOs exhibited no significant reduction in growth rateon corn straw compared to the wild-type strain. Functional characterizationof these AA9 LPMOs showed significant differences in oxidative regioselectivity,catalytic activity, and substrate specificity. Notably, MtLPMO9G exhibited dual catalytic activities, degrading cellulose andxylan through oxidation and hydrolysis reactions, respectively. Moreover,synergistic actions between AA9 LPMOs and GHs from various familieson enzymatic saccharification of corn straw with alkaline pretreatmentshowed that MtLPMO9G and MtLPMO9Hincreased the catalytic efficiency of cellulolytic GHs by 28 to 242%.These findings demonstrate great application potential of MtLPMO9G and MtLPMO9H in designing LPMO-containingcellulase cocktails for efficient enzymatic saccharification of lignocellulosicbiomass. Dual-functional MtLPMO9G and C1/C4-oxidizing MtLPMO9H significantlyincreased the catalytic efficiencyof cellulolytic GHs.

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