Knockdown of the cap 'n ' collar isoform C gene increases the susceptibility of Agrotis ipsilon to chlorantraniliprole and phoxim

文献类型： 外文期刊

第一作者： Xiao, Qing-Hua

作者： Xiao, Qing-Hua;Zhang, Jin;Li, Mao -Ye;Liu, Su;Li, Wu -Ye;Yu, Jia-Min;Liu, Dong-Yang;Peng, Jiang -Nan

作者机构：

关键词： Black cutworm; CncC; RNA interference; Glutathione S -transferase; Insecticide detoxification

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.5； 五年影响因子：1.5 ）

ISSN： 1226-8615

年卷期： 2024 年 27 卷 2 期

页码：

收录情况： SCI

摘要： In insects, the transcription factor cap 'n ' collar isoform C (CncC) plays a critical role in the regulation of multiple genes involved in insecticide detoxification. Knockdown of CncC genes leads to increased susceptibility to different types of insecticides in several insect species. However, the CncC gene has not yet been fully characterized in the black cutworm Agrotis ipsilon , a notorious insect pest that causes severe damage to field crops. In this study, the CncC gene (designated AiCncC ) was identified from A. ipsilon . Exposure to a median lethal concentration (LC 50 ) of chlorantraniliprole (CAP) or phoxim (PHO) strongly increased the expression of AiCncC . Silencing of AiCncC by RNA interference significantly increased the susceptibility of A. ipsilon larvae to both insecticides. Moreover, CncC/Maf binding sites were predicted in the putative promoters of two glutathione Stransferase (GST) genes ( AiGSTe1 and AiGSTu1 ) involved in the detoxification of CAP and PHO. The transcription levels of AiGSTe1 and AiGSTu1 were dramatically decreased by silencing AiCncC . These findings indicate that AiCncC is associated with CAP and PHO susceptibility through the regulation of GST genes.

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