Autophagy promoted infectious kidney and spleen necrosis virus replication and decreased infectious virus yields in CPB cell line

文献类型： 外文期刊

第一作者： Li, Chen

作者： Li, Chen;Fu, Xiaozhe;Lin, Qiang;Liu, Lihui;Liang, Hongru;Huang, Zhibin;Li, Ningqiu;Li, Chen;Fu, Xiaozhe;Lin, Qiang;Liu, Lihui;Liang, Hongru;Li, Ningqiu

作者机构：

关键词： Siniperca chuatsi; ISKNV; Autophagy; LC3B; Antiviral response

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2017 年 60 卷

页码：

收录情况： SCI

摘要： Autophagy plays important functions in viral replication and pathogenesis. In this study, we investigated the role of autophagy in the replication of infectious kidney and spleen necrosis virus (ISKNV), an agent that has caused devastating losses in Chinese perch (Siniperca chuatsi) industry. We found that ISKNV infection triggered the complete autophagic process, as demonstrated by microtubule-associated protein 1 light chain 3B II (LO3B-II) conversion, an increased accumulation of punctate GFP-LC3-expressing cells, a higher number of autophagosome-double-membrane vesicles in the cytoplasm, and increased levels of autophagic flux in CPB cells. Then, we investigated the role of autophagy in the process of ISKNV replication. Results showed that inducing autophagy by rapamycin promoted ISKNV replication and proteins synthesis but decreased extracellular virus yields. While, blocking autophagosome-lysosome fusion by chloroquine (CQ) promoted infectious virus yields in culture supernatant. These results offer insight into the complex interactions between ISKNV and host cell, providing new insights into viral pathogenesis and antiviral treatment strategies. (C) 2016 Elsevier Ltd. All rights reserved.

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