Purification and Characterization of a New Alginate Lyase from Marine Bacterium Vibrio sp SY08

文献类型： 外文期刊

第一作者： Li, Shangyong

作者： Li, Shangyong;Wang, Linna;Hao, Jianhua;Xing, Mengxin;Sun, Jingjing;Sun, Mi;Hao, Jianhua;Sun, Mi

作者机构：

关键词： alginate lyase; thermostability; unsaturated alginate disaccharides; Vibrio sp.

期刊名称：MARINE DRUGS （ 影响因子：5.118； 五年影响因子：5.951 ）

ISSN： 1660-3397

年卷期： 2017 年 15 卷 1 期

页码：

收录情况： SCI

摘要： Unsaturated alginate disaccharides (UADs), enzymatically derived from the degradation of alginate polymers, are considered powerful antioxidants. In this study, a new high UAD-producing alginate lyase, AlySY08, has been purified from the marine bacterium Vibrio sp. SY08. AlySY08, with a molecular weight of about 33 kDa and a specific activity of 1070.2 U/mg, showed the highest activity at 40 degrees C in phosphate buffer at pH 7.6. The enzyme was stable over a broad pH range (6.0-9.0) and retained about 75% activity after incubation at 40 degrees C for 2 h. Moreover, the enzyme was active in the absence of salt ions and its activity was enhanced by the addition of NaCl and KCl. AlySY08 resulted in an endo-type alginate lyase that degrades both polyM and polyG blocks, yielding UADs as the main product (81.4% of total products). All these features made AlySY08 a promising candidate for industrial applications in the production of antioxidants from alginate polysaccharides.

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