Characterization, Tissue-Specific and Developmental Stage Expression of Somatostatin in Coilia nasus

文献类型： 外文期刊

第一作者： Yang, Siyu

作者： Yang, Siyu;Xu, Pao;Du, Fukuan;Xu, Pao

作者机构：

期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）

ISSN： 0030-9923

年卷期： 2017 年 49 卷 1 期

页码：

收录情况： SCI

摘要： Estuarine tapertail anchovy (Coilia nasus) is a rare and endangered species and also an important resource with high economic value. Somatostatm (SS) is a neuropeptide family which effects growth, development and metabolism In this study, full-length of one type of SS eDNA from C. nasus was synthesized, cloned and sequenced This SS eDNA encodes a protein with 114 amino acids that contains the SS14 sequence at its C-termmus. This putative peptide is identical to that generated by the SSI gene m other vertebrates. Tissue distribution of C. nasus SSI mRNA was analyzed by real-time polymerase chain reaction (PCR), which demonstrated high expression level in the brain During embryogenesis, SSI mRNA was detected during early-stage embryonic development, decreased during subsequent developmental stages then increased gradually from the stage of midgastrula onward This study provides some basic evidence that SSI may play a role in growth, development and metabolism in C. nasus, and provides a basis for further study of SS neuropeptide family in C. nasus

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