A farnesyl pyrophosphate synthase gene expressed in pollen functions in S-RNase-independent unilateral incompatibility

文献类型： 外文期刊

第一作者： Qin, Xiaoqiong

作者： Qin, Xiaoqiong;Li, Wentao;Liu, Yang;Tan, Meilian;Chetelat, Roger T.;Ganal, Martin;Li, Wentao;Liu, Yang;Tan, Meilian

作者机构：

关键词： unilateral incompatibility; farnesyl pyrophosphate synthase; transmission ratio distortion; Solanum lycopersicum; Solanum pennellii

期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）

ISSN： 0960-7412

年卷期： 2018 年 93 卷 3 期

页码：

收录情况： SCI

摘要： Multiple independent and overlapping pollen rejection pathways contribute to unilateral interspecific incompatibility (UI). In crosses between tomato species, pollen rejection usually occurs when the female parent is self-incompatible (SI) and the male parent self-compatible (SC) (the 'SI x SC rule'). Additional, as yet unknown, UI mechanisms are independent of self-incompatibility and contribute to UI between SC species or populations. We identified a major quantitative trait locus on chromosome 10 (ui10.1) which affects pollen-side UI responses in crosses between cultivated tomato, Solanum lycopersicum, and Solanum pennellii LA0716, both of which are SC and lack S-RNase, the pistil determinant of S-specificity in Solanaceae. Here we show that ui10.1 is a farnesyl pyrophosphate synthase gene (FPS2) expressed in pollen. Expression is about 18-fold higher in pollen of S. pennellii than in S. lycopersicum. Pollen with the hypomorphic S. lycopersicum allele is selectively eliminated on pistils of the F-1 hybrid, leading to transmission ratio distortion in the F-2 progeny. CRISPR/Cas9-generated knockout mutants (fps2) in S. pennellii LA0716 are self-sterile due to pollen rejection, but mutant pollen is fully functional on pistils of S. lycopersicum. F-2 progeny of S. lycopersicum x S. pennellii (fps2) show reversed transmission ratio distortion due to selective elimination of pollen bearing the knockout allele. Overexpression of FPS2 in S. lycopersicum pollen rescues the pollen elimination phenotype. FPS2-based pollen selectivity does not involve S-RNase and has not been previously linked to UI. Our results point to an entirely new mechanism of interspecific pollen rejection in plants.

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