文献类型： 外文期刊

第一作者： Liu, Z. -Z.

作者： Liu, Z. -Z.;Zhao, F. -R.;Gao, S. -D.;Shao, J. -J.;Zhang, Y. -G.;Chang, H. -Y.;Liu, Z. -Z.;Gao, S. -D.;Shao, J. -J.;Zhang, Y. -G.;Chang, H. -Y.

作者机构：

关键词： 3Aepitope-3Bepitope; chemiluminescence immunoassay; diagnosis; foot-and-mouth disease virus

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）

ISSN： 1865-1674

年卷期： 2018 年 65 卷 2 期

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收录情况： SCI

摘要： The contamination of inactivated vaccine with non-structural proteins (NSPs) leads to a high false-positive rate, which is a substantial barrier to accurately differentiate foot-and-mouth disease virus (FMDV)-infected animals from vaccinated animals. To address this problem, a new chemiluminescence immunoassay (CLIA) method was developed to detect antibodies targeting the two recombinant epitope-based proteins located in 3A and 3B. The 3Aepitp-3Bepitp CLIA exhibited a diagnostic sensitivity of 94.0% and a diagnostic specificity of 97.5% for the detection of serum samples (naive bovines, n=52, vaccinated bovines, n=422, infected bovines, n=116) from animals with known status. The CLIA method also had a concordance rate of 88.1% with the PrioCHECK FMDV NSP ELISA based on the detection of 270 serum samples from the field. Importantly, the 3Aepitp-3Bepitp CLIA produced no false-positives when used to detect FMDV in samples from bovines that had been vaccinated up to five times, and it was demonstrated a low false-positive rate when the bovines had been vaccinated up to ten (2.15%) and fifteen times (5.93%). Therefore, the 3Aepitp-3Bepitp CLIA detects FMDV in samples from frequently vaccinated bovines with high accuracy and represents an alternative method to differentiate FMDV-infected and vaccinated bovines.

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