A paper-based competitive lateral flow immunoassay for multi beta-agonist residues by using a single monoclonal antibody labelled with red fluorescent nanoparticles

文献类型： 外文期刊

第一作者： Wang, Ruiguo

作者： Wang, Ruiguo;Zhang, Wei;Wang, Peilong;Su, Xiaoou;Wang, Ruiguo;Zhang, Wei;Wang, Peilong;Su, Xiaoou

作者机构：

关键词： beta-Agonist; Fluorescent nanoparticles; Paper-based assay; Immunoassay; Multi residues; Pork tissue

期刊名称：MICROCHIMICA ACTA （ 影响因子：5.833； 五年影响因子：5.357 ）

ISSN： 0026-3672

年卷期： 2018 年 185 卷 3 期

页码：

收录情况： SCI

摘要： An ultrasensitive paper based lateral flow assay is described for rapid and simultaneous fluorometric detection of several beta-agonists including clenbuterol and its chemical analogues (mabuterol, brombuterol, cimaterol, cimbuterol, bromchlorbuterol and banbuterol). A nonspecific monoclonal antibody (mAb) against clenbuterol and its analogues was prepared and employed in a competitive immunoassay where mAb conjugated to fluorescent nanoparticles and free beta-agonists compete for the binding sites. This enables rapid screening for the 7 beta-agonists in a single run that takes about 8 min. Detection limits for the seven beta-agonists are <50 pg g(-1) of pork. Recoveries ranged from 69.5% to 102.4%, and relative standard deviations were +/- 15%. The assay was applied to the analysis of both using spiked and unspiked pork for beta-agonists, and the results compare well to those obtained by HPLC-MS.

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