A perfect functional marker for the gene of intermediate amylose content Wx-in in rice (Oryza sativa L.)

文献类型： 外文期刊

第一作者： Zhou, Lei

作者： Zhou, Lei;Chen, Shaoyu;Yang, Guocai;Zha, Wenjun;Cai, Haiya;Li, Sanhe;Chen, Zhijun;Liu, Kai;Xu, Huashan;You, Aiqing;You, Aiqing

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关键词： Intermediate amylose content; waxy gene; marker-assisted selection; grain quality; rice

期刊名称：CROP BREEDING AND APPLIED BIOTECHNOLOGY （ 影响因子：1.282； 五年影响因子：1.493 ）

ISSN： 1984-7033

年卷期： 2018 年 18 卷 1 期

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收录情况： SCI

摘要： The amylose content (AC) of rice is a key determinant of the grain cooking and eating quality. The Waxy (Wx) gene, which encodes granule-bound starch synthase I (GBSSI), is the major gene controlling AC in rice. The A-C substitution in exon 6 (Ex6A/C) alters the amino acid codon from serine to tyrosine and is associated with intermediate-AC rice (Wx-in). In this paper, we reported a perfect functional marker for the SNP Ex6A/C of Wx-in based on polymerase chain reaction with confronting two-pair primers (PCR-CTPP) in a single-tube PCR assay. Specificity and applicability of the PCR assay were verified in a Chinese mini core collection (MCC) of Oryza sativa L. and a breeding population. We recommend the use of the simple, inexpensive assay for routine genotyping for intermediate AC in the breeding population or discrimination of Wx-in genotypes in rice germplasm.

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